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Serum prolidase activity and oxidant-antioxidant status in children with chronic hepatitis B virus infection

Velat Şen1, Ünal Uluca2, Aydın Ece3

  • 1Department of Pediatrics, Dicle University Medical School, Diyarbakir, Turkey. drvelatsen@hotmail.com.

Italian Journal of Pediatrics
|November 27, 2014
PubMed

Insights

Elevated prolidase activity and oxidative stress are linked to chronic hepatitis B (CHB) in children. These markers may help identify CHB and its progression.

Area of Science:

  • Biochemistry
  • Hepatology
  • Pediatrics

Background:

  • Chronic hepatitis B (CHB) is a global health concern with complications linked to collagen degradation.
  • Prolidase, an enzyme crucial for collagen breakdown, is implicated in these processes.
  • Investigating prolidase activity and oxidative stress in pediatric CHB is essential.

Purpose of the Study:

  • To evaluate serum prolidase enzyme activity (SPEA) in children with CHB.
  • To assess the oxidant-antioxidant status in pediatric CHB.
  • To determine the relationship between prolidase activity, oxidative stress, and CHB activity.

Main Methods:

  • A prospective case-control study involving 38 CHB patients, 31 inactive hepatitis B (IHB) patients, and 29 healthy controls.
  • Measurement of serum prolidase enzyme activity (SPEA), total antioxidant capacity (TAC), total oxidative activity (TOA), and malondialdehyde (MDA).
  • Calculation of the oxidative stress index (OSI) for all participants.

Main Results:

  • CHB patients exhibited significantly higher SPEA, TOA, OSI, and MDA levels compared to controls and IHB patients.
  • IHB patients showed lower TOA and OSI values than CHB patients.
  • SPEA demonstrated significant positive correlations with HBV-DNA and ALT levels.

Conclusions:

  • Serum prolidase activity serves as a reliable indicator for diagnosing CHB in children.
  • Increased oxidative stress is associated with the chronicity of hepatitis B infection.
  • Prolidase activity and oxidative stress markers warrant further investigation in pediatric CHB management.
Abstract

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