Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Cryo-electron Microscopy01:28

Cryo-electron Microscopy

4.7K
Conventional electron microscopy (EM) involves dehydration, fixation, and staining of biological samples, which distorts the native state of biological molecules and results in several artifacts. Also, the high-energy electron beam damages the sample and makes it difficult to obtain high-resolution images. These issues can be addressed using cryo-EM, which uses frozen samples and gentler electron beams. The technique was developed by Jacques Dubochet, Joachim Frank, and Richard Henderson, for...
4.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Cryopreservation of articular cartilage. Part 3: The liquidus-tracking method.

Cryobiology·2020
Same author

The relevance of ice crystal formation for the cryopreservation of tissues and organs.

Cryobiology·2020
Same author

Foreward. Special issue in memoriam: Armand M. Karow Jr., Ph.D. (1941-2007).

Cryobiology·2010
Same author

The relevance of ice crystal formation for the cryopreservation of tissues and organs.

Cryobiology·2010
Same author

On criticising Israel. A thick skin may not provide protection.

BMJ (Clinical research ed.)·2009
Same author

Reduction of cryoprotectant toxicity in cells in suspension by use of a sodium-free vehicle solution.

Cryobiology·2007
Same journal

Tracking Synthetic Adhesins on Bacterial Surfaces with Immunofluorescence Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Post-Selection Methods for Analyzing mRNA Display Selections and Optimization of Hits.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

High-Performance Computing in Tandem Mass Spectrometry (MS/MS) Peptide Identification.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Engineering and Adapting Disulfide-Containing Proteins to Enable Intracellular Functionality.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

AI-Driven Protein Research: From Prediction to Design.

Methods in molecular biology (Clifton, N.J.)·2026
Same journal

Methods for the In Vitro Selection of Protein and Peptide Libraries Using mRNA Display.

Methods in molecular biology (Clifton, N.J.)·2026
See all related articles

Related Experiment Video

Updated: Apr 20, 2026

Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats
11:10

Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats

Published on: August 5, 2011

31.6K

Principles of cryopreservation.

David E Pegg1

  • 1Department of Biology, University of York, York, UK, dep1york@gmail.com.

Methods in Molecular Biology (Clifton, N.J.)
|November 28, 2014
PubMed
Summary
This summary is machine-generated.

Cryopreservation uses low temperatures to preserve cells and tissues. Understanding freezing injury mechanisms and cryoprotectant properties is key to effective cell and tissue preservation.

More Related Videos

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives
08:46

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives

Published on: September 16, 2021

6.9K
Collection and Cryopreservation of Hamster Oocytes and Mouse Embryos
13:36

Collection and Cryopreservation of Hamster Oocytes and Mouse Embryos

Published on: March 27, 2009

17.6K

Related Experiment Videos

Last Updated: Apr 20, 2026

Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats
11:10

Cryopreservation of Preimplantation Embryos of Cattle, Sheep, and Goats

Published on: August 5, 2011

31.6K
Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives
08:46

Fertility Preservation Through Oocyte Vitrification: Clinical and Laboratory Perspectives

Published on: September 16, 2021

6.9K
Collection and Cryopreservation of Hamster Oocytes and Mouse Embryos
13:36

Collection and Cryopreservation of Hamster Oocytes and Mouse Embryos

Published on: March 27, 2009

17.6K

Area of Science:

  • Cryobiology
  • Biophysics
  • Cell Biology

Background:

  • Cryopreservation preserves biological structures using low temperatures.
  • Unprotected freezing is typically lethal due to ice formation and solute concentration.
  • Understanding freezing injury mechanisms is crucial for developing effective preservation methods.

Purpose of the Study:

  • Analyze mechanisms of freezing injury in cells and tissues.
  • Investigate the role of cooling rates and cryoprotectants in cell survival.
  • Explore vitrification as an alternative to ice-based cryopreservation.

Main Methods:

  • Analysis of biological effects of cooling and water freezing.
  • Evaluation of cryoprotectant properties (penetration, toxicity).
  • Prediction of cell survival based on membrane permeability and cooling rates.

Main Results:

  • Both intracellular and extracellular ice formation contribute to freezing injury.
  • Cryoprotectants reduce ice formation but must be non-toxic and cell-permeable.
  • Vitrification offers ice-free preservation but faces toxicity challenges.
  • Solute diffusion and osmosis significantly impact cryopreservation processes.

Conclusions:

  • Effective cryopreservation balances intracellular freezing risk with solute effects.
  • Optimizing cooling and warming rates is critical for cell survival.
  • Cryoprotectant delivery and removal dynamics influence preservation success.
  • Cryopreservation and vitrification methods have broad applications in biology and medicine.