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Laser scanning microscopy in cryobiology.

Frank Stracke1, Asger Kreiner-Møller, Heiko Zimmermann

  • 1Fraunhofer Institute for Biomedical Technology, Ensheimer Str. 48, 66386, St. Ingbert, Germany.

Methods in Molecular Biology (Clifton, N.J.)
|November 28, 2014
PubMed
Summary
This summary is machine-generated.

Advanced laser scanning microscopy techniques, including Raman and multiphoton imaging, offer powerful insights into cryobiology. These methods enable detailed chemical mapping and study of intracellular ice formation during cryopreservation.

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Area of Science:

  • Cryobiology
  • Microscopy
  • Spectroscopy

Background:

  • Laser scanning microscopy is a key technology in cryobiology.
  • It can be integrated with various imaging modalities like Raman spectroscopy and multiphoton imaging.

Purpose of the Study:

  • To describe advanced cryoimaging methods.
  • To highlight imaging modalities for studying cryopreservation.

Main Methods:

  • Multiphoton imaging for subcellular ice formation studies.
  • Raman imaging for chemical mapping of frozen samples.
  • Temperature-controlled sample holders for in-situ spectral collection during freezing and thawing.

Main Results:

  • Raman spectroscopy provides molecular vibration information.
  • Raman imaging localizes intra- and extracellular constituents and water forms in freeze-concentrated solutions.
  • Advanced methods allow detailed analysis of cryopreservation processes.

Conclusions:

  • Laser scanning microscopy with advanced modalities is crucial for cryobiology research.
  • These techniques provide unprecedented detail on sample behavior during cryopreservation.