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Lipoxygenase inhibitory activity of Cuspidaria pulchra and isolated compounds
Tavane A Alvarenga1, Camila S Bertanha, Pollyanna F de Oliveira
1a Núcleo de Pesquisa em Ciências Exatas e Tecnológicas, Universidade de Franca , Av. Dr. Armando Salles de Oliveira, 201, 14404-600 Franca , Brazil.
Abstract:
This work evaluated the in vitro inhibitory activity of the crude ethanolic extract from the aerial parts of Cuspidaria pulchra (Cham.) L.G. Lohmann against 15-lipoxygenase (15-LOX). The bioassay-guided fractionation of the n-butanol fraction, which displayed the highest activity, led to the isolation of three compounds: caffeoylcalleryanin (1), verbascoside (2) and 6-hydroxyluteolin-7-O-β-glucoside (3). Assessment of the ability of the isolated compounds to inhibit 15-LOX revealed that compounds 1, 2 and 3 exerted strong 15-LOX inhibitory activity; IC50 values were 1.59, 1.76 and 2.35 μM respectively. The XTT assay showed that none of the isolated compounds seemed to be significantly toxic.
