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Updated: Apr 20, 2026
Rapid and Efficient Generation of Neurons from Human Pluripotent Stem Cells in a Multititre Plate Format
Published on: March 5, 2013
A spatially and chemically defined platform for the uniform growth of human pluripotent stem cells
Steven J Jonas1, Jackelyn A Alva2, Wade Richardson3
1Department of Materials Science and Engineering, University of California Los Angeles, Los Angeles, California 90095, USA.
Abstract:
We report the design of a chemically defined platform engineered for the culture of human pluripotent stem cells (hPSCs) that supports the long-term maintenance of self-renewing hPSC populations in a more uniform manner than standard culture systems. Microcontact printing (μCP) of alkanethiol self-assembled monolayers (SAMs) was used to spatially direct hPSC adherence. This technique not only establishes control over hPSC colony size and shape but also preserves genetic stability and provides unprecedented uniformity in the pluripotency of hPSC populations that is quantitatively assessed in the present study.
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