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RAGE controls leukocyte adhesion in preterm and term infants
Kirsten Buschmann1, Raphaela Tschada2, Marie-Sophie Metzger3
1Department of Neonatology, University Hospital, 69120, Heidelberg, Germany. Kirsten.Buschmann@med.uni-heidelberg.de.
BMC Immunology
|November 28, 2014
Summary
Receptor of advanced glycation end products (RAGE) is vital for leukocyte adhesion in preterm and term infants. This molecule is crucial for immune cell function, impacting infection risk in newborns.
Area of Science:
- Immunology
- Neonatology
- Cellular Biology
Background:
- Preterm infants face high infection rates due to insufficient leukocyte recruitment.
- Receptor of advanced glycation end products (RAGE), a leukocyte adhesion molecule, is highly expressed during early development.
Purpose of the Study:
- To investigate the role of RAGE in leukocyte recruitment in preterm and term infants.
- To compare RAGE function in neonatal leukocytes with adult leukocytes.
Main Methods:
- Dynamic flow chamber experiments with leukocytes from premature and mature neonates and adults.
- Fluorescent microscopy for leukocyte adhesion analysis.
- ELISA for soluble RAGE (sRAGE) measurement and flow cytometry for β2-Integrin expression (Mac-1, LFA-1).
Main Results:
- Leukocyte adhesion functions increase significantly with gestational age.
- RAGE is crucial for leukocyte adhesion in all infant age groups, comparable to ICAM-1.
- High sRAGE levels in extremely premature infants decrease with gestational age, while β2-Integrin expression remains constant.
Conclusions:
- RAGE is a critical leukocyte adhesion molecule in both preterm and term infants.
- RAGE plays a significant role in neonatal immune responses and infection susceptibility.
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