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Published on: March 12, 2015
Foetithiophenes C-F, thiophene derivatives from the roots of Ferula foetida
Mahsa Chitsazian-Yazdi1, Sara Agnolet, Sybille Lorenz
1Biotechnology Research Center, School of Pharmacy, Mashhad University of Medical Sciences , Mashhad , Iran .
Context:
Ferula foetida Regel (Apiaceae) is an Iranian medicinal plant with various biological activities including antispasmodic and anthelmintic.
Objective:
The sulfur compounds from the roots of F. foetida were isolated and characterized to test their cytotoxic and antimicrobial activities.
Materials And Methods:
The methanolic extract of the roots of F. foetida was fractionated using silica column chromatography. The components of each fraction were further purified using RP-HPLC. Their structures were elucidated by 1- and 2-D NMR spectroscopy as well as HREIMS. Their cytotoxic and antimicrobial activities were evaluated using Alamar Blue assay and broth microdilution method, respectively.
Results:
Four new thiophene derivatives, namely foetithiophenes C-F (3-6), together with four known compounds, foetithiophenes A (1) and B (2), coniferaldehyde, and sinapic aldehyde, were isolated from the roots of F. foetida. Antimicrobial activities were observed in particular against the Gram-positive bacteria. The best antimicrobial activity was observed for compound 6 against B. cereus with a MIC value 50 µg/mL. The tested compounds did not show cytotoxic properties against MCF-7 and K562 cells.
Conclusion:
Four new thiophene derivatives including foetithiophenes C-F (3-6) were characterized from the roots of F. foetida. Foetithiophene F (6) exhibited the most potent activity against the Gram-positive bacteria B. cereus.
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