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Discovering human RNA aptamers by structure-based bioinformatics and genome-based in vitro selection
Bao Ho1, Julio Polanco1, Randi Jimenez1
1Department of Pharmaceutical Sciences, University of California, Irvine, California, USA; Department of Molecular Biology and Biochemistry, University of California, Irvine, California, USA; Department of Chemistry, University of California, Irvine, California, USA.
We designed a human genomic library for in vitro selection to discover RNA aptamers. These aptamers can bind specific metabolites, aiding in the discovery of similar RNA molecules through structure-based searches.
Area of Science:
- Molecular Biology
- Biochemistry
- Genomics
Background:
- In vitro selection and structure-based searches are key for discovering complex RNA molecules.
- RNA aptamers with high affinity and specificity for metabolites are valuable tools.
Purpose of the Study:
- To design a human genomic library for RNA aptamer discovery.
- To enable structure-based searches for analogous aptamers using selected RNA structures.
Main Methods:
- Design of a human genomic library as a DNA template.
- In vitro selection to identify RNA aptamers.
- Structure determination of selected RNA aptamers.
Main Results:
- A functional human genomic library was created.
- The library facilitates in vitro selection of RNA aptamers.
- Structural data from selected aptamers can guide further database searches.
Conclusions:
- The designed genomic library is effective for discovering RNA aptamers.
- This approach combines in vitro selection and structure-based methods for broader aptamer discovery.
- Enables identification of novel RNA molecules targeting metabolites.
