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Labeling DNA Probes

Labeling DNA Probes

DNA probes are fragments of DNA labeled with a reporter tag to enable their detection or purification. The resulting labeled DNA probes can then hybridize to target nucleic acid sequences through complementary base-pairing, and may be used to recover or identify these regions.
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Updated: Mar 11, 2026

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Real-time Imaging of Single Engineered RNA Transcripts in Living Cells Using Ratiometric Bimolecular Beacons

Published on: August 6, 2014

Bioluminescent Probes for Multiplexed RNA Imaging.

Erin B Fuller¹, Kyle H Cole², Lila P Halbers³

¹Department of Chemistry, University of California Irvine, Irvine, California 92617, United States.

Journal of the American Chemical Society

|March 10, 2026

Summary

This summary is machine-generated.

Researchers developed novel bioluminescent RNA probes called RNA lanterns for multiplexed RNA detection. These probes enable sensitive, continuous visualization of multiple RNA targets within living cells without excitation light.

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Area of Science:

Molecular Biology
Biotechnology
Cell Biology

Background:

Understanding RNA biology necessitates methods for visualizing RNA transcripts and their interactions in real-time within physiological environments.
Current technologies are limited in their ability to continuously monitor multiple RNA species simultaneously in living systems.
Bioluminescent probes offer a promising alternative due to their sensitivity and lack of requirement for excitation light, enabling noninvasive imaging.

Purpose of the Study:

To develop and validate a novel system of bioluminescent probes for multiplexed RNA detection in living systems.
To create orthogonal RNA probes capable of distinguishing and reporting on multiple RNA targets concurrently.
To expand the toolkit for RNA imaging and facilitate the study of RNA dynamics in vivo.

Main Methods:

Designed and synthesized a panel of structured RNA tags and complementary luciferase fragments (RNA lanterns).
Developed three orthogonal tag-lantern combinations utilizing known RNA-binding proteins for specific target recognition.
Optimized probe performance in vitro for selective transcript detection and validated in cultured cells for multitarget imaging.

Main Results:

Successfully created RNA lanterns that assemble and emit light only when brought into proximity by specific RNA tags.
Demonstrated selective detection of distinct RNA targets using orthogonal tag-lantern combinations.
Achieved multitarget RNA imaging in cultured cells with good subcellular resolution, showcasing the probes' efficacy in a biological context.

Conclusions:

The developed RNA lanterns provide a novel and sensitive method for multiplexed RNA detection.
These probes overcome limitations of existing technologies by enabling continuous, noninvasive monitoring of multiple RNA species in living systems.
The RNA lantern system significantly expands the capabilities for RNA imaging, supporting future research in RNA biology and dynamics.