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Hybridoma Technology

Hybridoma Technology

Hybridoma technology is used for the large-scale production of monoclonal antibodies. Monoclonal antibodies bind to only a single antigenic determinant or epitope. Such antibodies are used in research, diagnostics, and disease therapy. The hybridoma technology established in 1975 by Georges Köhler and Cesar Milstein was awarded the Nobel Prize in Medicine in 1984 for revolutionizing research and therapy.
Hybridoma Selection
Commonly used fusion techniques — electroporation, polyethylene glycol...

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Related Experiment Video

Updated: Jun 17, 2026

Initial Evaluation of Antibody-conjugates Modified with Viral-derived Peptides for Increasing Cellular Accumulation and Improving Tumor Targeting

Initial Evaluation of Antibody-conjugates Modified with Viral-derived Peptides for Increasing Cellular Accumulation and Improving Tumor Targeting

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A novel imaging-based multi-sample viscometry application for advancing monoclonal antibody development.

Karen L Lopez¹, Francesco Palomba¹, Marissa Mock²

¹Laboratory for Fluorescence Dynamics, Biomedical Engineering Department, University of California, Irvine, CA, United States of America.

Methods and Applications in Fluorescence

|April 10, 2026

Summary

A new imaging technique uses fluorescent beads and single particle tracking to rapidly measure monoclonal antibody viscosity. This high-throughput method requires minimal sample volume, accelerating therapeutic development.

Keywords:

fluorescence microscopy monoclonal antibody viscometers

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Last Updated: Jun 17, 2026

Initial Evaluation of Antibody-conjugates Modified with Viral-derived Peptides for Increasing Cellular Accumulation and Improving Tumor Targeting

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Published on: March 8, 2018

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Area of Science:

Biotechnology
Biophysics
Analytical Chemistry

Background:

Therapeutic monoclonal antibody (mAb) development is critical but faces bottlenecks in sample testing.
Viscosity is a key mAb property impacting drug delivery and patient experience.
Existing viscometry methods are slow, require large sample volumes, and lack automation.

Purpose of the Study:

To develop and validate a novel, high-throughput, low-volume imaging-based viscometry method for mAb solutions.
To address the limitations of traditional viscometry techniques in mAb development.

Main Methods:

Integration of a wide-field camera, fluorescence microscope, and Python-based Single Particle Tracking (SPT) software.
Utilized 200 nm yellow-green fluorescent polystyrene beads as tracers in low-volume (2 μL) samples.
Validated with Bovine Serum Albumin (BSA), viscosity standards, and blinded IgG1/IgG2 solutions (≤150 mg/mL).

Main Results:

The SPT method achieved rapid, accurate macroviscosity measurements.
Blinded analysis of IgG solutions showed a high linear fit (R² = 0.97) with an average error of 2.1 cP compared to unblinded values.
Demonstrated high-throughput capability and precision.

Conclusions:

The novel imaging-based viscometry platform significantly streamlines mAb development by overcoming throughput and sample volume limitations.
This method offers a cost-effective and efficient solution for a pressing challenge in the biopharmaceutical industry.
Enables faster optimization of mAb properties for improved therapeutic agents.