Related Experiment Video
Updated: Apr 20, 2026

A Femtoliter Droplet Array for Massively Parallel Protein Synthesis from Single DNA Molecules
Published on: June 20, 2020
Precise quantitative addition of multiple reagents into droplets in sequence using glass fiber-induced droplet
1Single-Cell Center, CAS Key Laboratory of Biofuels and Shandong Key Laboratory of Energy Genetics, Qingdao Institute of Bioenergy and Bioprocess Technology, Chinese Academy of Sciences, Qingdao, Shandong 266101, China. mabo@qibebt.ac.cn xujian@qibebt.ac.cn.
Abstract:
Precise quantitative addition of multiple reagents into droplets in sequence is still a bottleneck in droplet-based analysis. To address this issue, we presented a simple and robust glass fiber-induced droplet coalescence method. The hydrophilic glass fiber embedded in the microchannels can induce the deformation of droplets and trigger the coalescence. Serial addition of reagents with controlled volumes was performed by this method without the requirement for an external power source.

