Iron speciation by microsequential injection solid phase spectrometry using 3-hydroxy-1(H)-2-methyl-4-pyridinone as...

Area of Science:

• Analytical Chemistry
• Environmental Chemistry

Background:

• Accurate iron speciation is crucial for understanding water quality.
• Traditional methods can be time-consuming and require extensive sample preparation.

Purpose of the Study:

• To develop a novel microsequential injection lab-on-valve solid phase spectrophotometry (µSI-LOV-SPS) method for iron speciation.
• To utilize a new ligand, 3-hydroxy-1(H)-2-methyl-4-pyridinone, for iron analysis.

Main Methods:

• Iron was retained on Nitrilotriacetic Acid Superflow (NTA) resin in a reusable column.
• The ligand acted as both a chromogenic and eluting reagent.
• Direct sample introduction was employed for fresh and high salinity coastal waters.

Main Results:

• The method achieved iron detection in the range of 20.0-100 µg/L with a limit of detection (LOD) of 9 µg/L.
• Analyzed reference samples showed relative deviations <10%.
• Bathing water analysis yielded results comparable to atomic absorption spectrophotometry (relative deviations <6%).

Conclusions:

• The developed µSI-LOV-SPS method offers a sensitive and accurate approach for iron speciation.
• This method enables direct analysis of diverse water matrices with reduced effluent production.
• The use of the novel ligand proved effective for both iron retention and elution.