Fabricating polystyrene fiber-dehydrogenase assemble as a functional biocatalyst

Area of Science:

• Biotechnology
• Materials Science
• Biocatalysis

Background:

• Enzyme immobilization on nanostructured materials improves stability, activation, and reusability.
• Bioconversion of CO2 to renewable bioenergy requires efficient catalytic processes.

Purpose of the Study:

• To develop polystyrene fiber-enzyme assemblies for catalyzing formaldehyde to methanol dehydrogenation.
• To enhance enzyme stabilization, activation, and reusability for CO2 bioconversion.

Main Methods:

• Fabrication and modification of electrospun polystyrene fibers.
• Immobilization of dehydrogenase onto the modified fibers.
• Evaluation of biochemical activities and storage stability of the fiber-enzyme assemblies.

Main Results:

• Polystyrene fibers demonstrated high capability for immobilizing dehydrogenase.
• Nitriation and silanization treatments optimized enzyme activity and long-term storage stability.
• The developed fiber-enzyme assemblies showed promising results for large-scale applications.

Conclusions:

• Enzyme immobilization on nanostructured polystyrene fibers is effective for enhancing enzyme properties.
• The developed fiber-enzyme assemblies are suitable for the bioconversion of CO2 to methanol.
• This approach holds significant potential for future renewable bioenergy applications.