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Updated: Apr 20, 2026

Development of an Electrochemical DNA Biosensor to Detect a Foodborne Pathogen
Published on: June 3, 2018
Electrochemical biosensor for DNA demethylase detection based on demethylation triggered endonuclease BstUI and
Huanshun Yin1, Zhiqing Yang1, Bingchen Li1
1College of Chemistry and Material Science, Shandong Agricultural University, 271018 Taian, Shandong, PR China.
Abstract:
Herein, an electrochemical biosensor was fabricated for DNA demethylase detection based on DNA demethylation triggered endonuclease BstUI and Exonuclease III digestion. After the double-strand DNA was demethylated, it can be further digested by BstUI and formed a blunt end at the electrode surface. Then, the remained fragment of DNA-DNA duplex was further cleaved by exonuclease III and led to increased electrochemical signal. Based on this detection strategy, the biosensor showed high sensitivity with low detection limit of 0.15ng/mL. Moreover, the developed method also presented high selectivity and acceptable reproducibility. This work provides a novel detection platform for DNA demethylase detection.
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