Selenium containing conducting polymer based pyranose oxidase biosensor for glucose detection.

Area of Science:

• Electrochemistry
• Materials Science
• Biotechnology

Background:

• Glucose detection is crucial in various fields, including healthcare and food analysis.
• Development of sensitive and selective biosensors is an ongoing area of research.
• Conducting polymers offer promising platforms for biosensor development due to their unique electrochemical properties.

Purpose of the Study:

• To develop a novel amperometric pyranose oxidase (PyOx) biosensor for glucose detection.
• To synthesize and characterize a selenium-containing conducting polymer, poly(BSeTT), for biosensor applications.
• To evaluate the performance of the developed biosensor for glucose determination in real samples.

Main Methods:

• Synthesis of poly(BSeTT) via electropolymerization on a gold electrode.
• Immobilization of PyOx enzyme onto the polymer matrix using physical adsorption.
• Amperometric detection of consumed oxygen at -0.7 V vs Ag reference electrode.
• Electrochemical characterization using SEM, EIS, and CV techniques.

Main Results:

• The poly(BSeTT) matrix exhibited suitable properties for enzyme immobilization and glucose detection.
• The developed biosensor showed a low limit of detection (LOD) of 3.3 × 10(-4) nM and a sensitivity of 6.4 nA/mM cm(2).
• The biosensor was successfully applied for the determination of glucose in beverage samples.

Conclusions:

• A novel and effective amperometric PyOx biosensor based on a selenium-containing conducting polymer was successfully developed.
• The poly(BSeTT) conducting polymer serves as a promising matrix for enzyme immobilization in biosensor construction.
• The developed biosensor offers a sensitive and reliable method for glucose detection in beverages.