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Updated: Jun 7, 2026

Analysis of LINE-1 Retrotransposition at the Single Nucleus Level
Published on: April 23, 2016
A recombination hotspot in the LTR of a mouse retrotransposon identified in an in vitro system
W Edelmann1, B Kröger, M Goller
1Institut für Virologie und Immunbiologie, Universität Würzburg, Federal Republic of Germany.
Abstract:
The recombinational frequency between two long terminal repeat elements (LTR-IS) of a mouse retrotransposon was about 13 times higher, compared with that of two control DNA sequences in extracts from mouse testes, but not in extracts from ascites cells. Deletion of a 37 bp region from the LTR-IS element strongly suppresses its recombinational activity. This 37 bp region encompasses an area of potentially single-stranded DNA and interacts with at least two nuclear proteins. One of them binds sequence-specifically to single-stranded DNA and is present in both types of extracts. Another protein(s) binds to dsDNA at the motif TGGAAATCCCC and is absent in extracts from testes. Our results suggest that a cis-acting DNA sequence within the 504 bp LTR-IS element is responsible for its high recombinational activity in vitro, and they further support the previous suggestion that the LTR-IS elements are meiotic recombinational hotspots in vivo.
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