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High throughput quantification of prohibited substances in plasma using thin film solid phase microextraction.

Nathaly Reyes-Garcés1, Barbara Bojko1, Janusz Pawliszyn1

  • 1Department of Chemistry, University of Waterloo, Ontario N2L 3G1, Canada.

Journal of Chromatography. A
|December 3, 2014
PubMed
Summary

This study introduces an automated thin film solid phase microextraction (SPME) method for rapid, high-throughput bioanalysis. The developed approach efficiently isolates multiple compounds from plasma with minimal matrix effects, achieving fast sample preparation times.

Keywords:
High throughputPlasmaProhibited substancesSample preparationSolid phase microextractionThin film

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Area of Science:

  • Analytical Chemistry
  • Biomolecular Analysis

Background:

  • High-throughput sample preparation is crucial for efficient bioanalysis.
  • Matrix interferences in biological samples can negatively impact analytical results.
  • Existing methods often lack speed or efficiency in removing interfering substances.

Purpose of the Study:

  • To develop an automated, high-throughput sample preparation method for analyzing multiple prohibited substances in plasma.
  • To utilize thin film solid phase microextraction (SPME) for efficient compound isolation.
  • To minimize matrix effects and ensure instrumental performance.

Main Methods:

  • An automated system employing 96 thin film SPME devices was developed.
  • A biocompatible SPME phase using hydrophilic-lipophilic balance particles immobilized with polyacrylonitrile (PAN) was utilized.
  • Experimental conditions including pre-conditioning, extraction, wash, and desorption were optimized.

Main Results:

  • The method demonstrated satisfactory extraction for 25 compounds with a wide polarity range (logP -2 to 6.8).
  • Preparation time was reduced to approximately 1.5 minutes per sample.
  • Excellent matrix effect mitigation was observed, with 24 out of 25 analytes showing values between 100-120%.

Conclusions:

  • The automated thin film SPME method offers a simple, fast, and efficient solution for high-throughput bioanalysis.
  • The PAN-based SPME coating provides biocompatibility and effective isolation of diverse compounds from plasma.
  • The validated method shows high linearity, accuracy, precision, and low limits of quantitation, making it suitable for routine analysis.