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Updated: Oct 22, 2026

Resolving Affinity Purified Protein Complexes by Blue Native PAGE and Protein Correlation Profiling
Published on: April 1, 2017
Complementary information from isotachophoresis and high-performance liquid chromatography in peptide analysis
P S Janssen1, J W van Nispen, M J van Zeeland
1AKZO Pharma Division, Organon International B.V., Oss, The Netherlands.
Abstract:
Reversed-phase high-performance liquid chromatography is a valuable analytical technique to support the synthesis, isolation and purification of peptides, as is illustrated by some critical separations. In addition to this technique, capillary isotachophoresis can give useful information on the purity determination of peptides and on the presence of ionic compounds of a non-peptidic nature. With regard to the latter aspect, isotachophoresis proved to be a suitable technique as a check on the effective removal of salts after preparative high-performance liquid chromatography.
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