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Urinary oestriol-16-glucuronide determined by "on-line" liquid chromatography
U Tiel1, P Heilmann, R Rejaibi
1Städtisches Krankenhaus Berlin-Spandau, Abteilung für Laboratoriumsmedizin, Berlin, F.R.G.
Journal of Clinical Chemistry and Clinical Biochemistry. Zeitschrift Fur Klinische Chemie Und Klinische Biochemie
|April 1, 1989
Summary
A new automated method accurately measures oestriol-16-glucuronide in urine, simplifying fetal well-being monitoring. This technique bypasses hydrolysis, offering a faster alternative for clinical assessments.
Area of Science:
- Clinical Chemistry
- Biochemistry
- Analytical Chemistry
Background:
- Accurate monitoring of fetal well-being during pregnancy is crucial.
- Oestriol-16-glucuronide is a key urinary marker for assessing fetal health.
- Existing methods for oestriol-16-glucuronide determination can be time-consuming, often requiring hydrolysis.
Purpose of the Study:
- To develop and validate a fully automated method for the specific assessment of oestriol-16-glucuronide in urine.
- To establish the reliability and sensitivity of the automated assay.
- To evaluate the method's utility for biochemical monitoring of fetal well-being, particularly under emergency conditions.
Main Methods:
- An "on-line" automated system integrating sample preparation (enrichment, pre-purification, focusing, injection) with high-performance liquid chromatography (HPLC) and fluorimetric detection.
- External calibration was employed due to negligible analyte losses.
- Assay variability (intra- and inter-assay) and sensitivity were determined.
Main Results:
- The automated method demonstrated high specificity and negligible losses of oestriol-16-glucuronide.
- Low coefficients of variation (4.44% intra-assay, 7.85% inter-assay) indicate good reproducibility.
- Assay sensitivity was found to be 430 nmol/l.
- Excretion rates in pregnancy urines correlated well (r = 0.957) with total urinary oestriol measured by conventional HPLC after hydrolysis.
- Reference ranges for different gestational weeks were established.
Conclusions:
- The fully automated HPLC method provides a specific, sensitive, and reproducible means for quantifying oestriol-16-glucuronide in urine.
- The method eliminates the need for a hydrolysis step, significantly reducing assay time.
- This automated approach is well-suited for rapid biochemical monitoring of fetal well-being, especially in emergency situations.