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Purification of Schwann cells

Amanda Brosius Lutz1

  • 1Stanford University School of Medicine, Department of Neurobiology, Stanford, California 94305.

Cold Spring Harbor Protocols
|December 3, 2014
PubMed

This article introduces methods for the acute purification and primary culture of Schwann cells from the mouse sciatic nerve. Immunopanning can be used to isolate Schwann cells from intact nerves during early postnatal development as well as to purify Schwann cells from adult nerves following sciatic nerve injury. These methods facilitate the exploration of mouse Schwann cell biology in the healthy and injured peripheral nerve.

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