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Related Concept Videos

Aggregates Classification01:29

Aggregates Classification

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Aggregate classification is generally based on its size, petrographic characteristics, weight, and source. Size classification ranges from coarse to fine aggregates, defined by the size of the particles. Coarse aggregates are particles that do not pass through ASTM sieve No. 4, and aggregates that pass through the sieve are fine aggregates.
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Response Surface Methodology (RSM) is a collection of statistical and mathematical techniques used to develop, improve, and optimize processes. It is particularly valuable when many input variables or factors potentially influence a response variable.
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Bonding and Strength of Aggregate01:12

Bonding and Strength of Aggregate

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The bond between aggregate particles and the cement matrix is significantly influenced by the shape and surface texture of the aggregates. High-strength concretes benefit from a rougher texture, which leads to stronger bonding due to greater adhesion. Angular aggregates with larger surface areas also enhance this bond. The bonding quality, however, is complex to assess as no universally accepted test exists. Good bonding is indicated when a crushed concrete specimen shows some aggregate...
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Specific Gravity of Aggregate01:19

Specific Gravity of Aggregate

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Aggregates typically contain pores, which can be either permeable or impermeable. Considering the pores in the aggregates, the specific gravity of aggregates is defined in three different forms, namely, bulk or gross specific gravity, apparent specific gravity, and absolute specific gravity.
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Bulk Density of Aggregate01:22

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Bulk density refers to the mass of aggregate particles that would fill a unit volume. The concept of bulk density originates from the inability to pack aggregate particles in a manner that completely eliminates void spaces. Hence, the term bulk refers to the volume that encompasses both the aggregates and the voids. This measurement is crucial when aggregates are batched by volume and is used to convert quantities by mass to volume.
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Preplaced Aggregate Concrete01:29

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Preplaced aggregate concrete is ideal for construction environments that are not easily accessible. The process begins by properly wetting the gap-graded coarse aggregates to remove the dirt, then placing it in the form and compacting it. Voids are filled with a mortar mix pumped under pressure through slotted pipes. This mortar typically consists of Portland cement, pozzolan, fine aggregates, water, and a fluidizing aid. The pozzolan helps reduce bleeding and segregation while improving the...
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In Vitro Assay for Studying the Aggregation of Tau Protein and Drug Screening
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A screening methodology for purifying proteins with aggregation problems.

Mario Lebendiker1, Michal Maes, Assaf Friedler

  • 1Protein Purification Facility, Wolfson Centre for Applied Structural Biology, The Hebrew University of Jerusalem, Safra Campus, Givat Ram, Jerusalem, 91904, Israel, mario.l@mail.huji.ac.il.

Methods in Molecular Biology (Clifton, N.J.)
|December 3, 2014
PubMed
Summary
This summary is machine-generated.

This study presents a simple laboratory method for purifying proteins prone to aggregation, particularly intrinsically disordered proteins (IDPs). The strategy focuses on maintaining protein solubility and stability during purification and storage.

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Area of Science:

  • Biochemistry
  • Protein Chemistry
  • Molecular Biology

Background:

  • Protein aggregation and insolubility present significant challenges during protein expression and purification.
  • Intrinsically disordered proteins (IDPs) and those with intrinsically disordered regions (IDRs) are particularly susceptible to aggregation due to their flexible structures.

Purpose of the Study:

  • To describe a practical and cost-effective strategy for purifying proteins prone to aggregation.
  • To provide guidelines for maintaining protein solubility and stability during purification and storage.

Main Methods:

  • Development of a purification strategy suitable for small laboratories without specialized equipment.
  • Focus on factors influencing protein solubility and stability.
  • Emphasis on early elimination of protein aggregates.

Main Results:

  • A methodology for purifying aggregation-prone proteins, including IDPs and IDRs, has been established.
  • The procedure is adaptable for various proteins exhibiting aggregation tendencies during expression or purification.

Conclusions:

  • The described method offers a viable solution for researchers facing challenges with protein aggregation.
  • This approach facilitates the purification of difficult protein targets in resource-limited settings.