Means to optimize protein expression in transgenic plants.

Area of Science:

• Biotechnology
• Molecular Biology
• Plant Sciences

Background:

• Protein production relies on diverse expression systems, including bacteria, mammalian cells, and transgenic plants.
• Transgenic plants have emerged as a competitive platform for biopharmaceutical protein production.
• Gene engineering offers methods to enhance yields in recombinant protein production.

Purpose of the Study:

• To review and discuss the current status of recombinant protein production in transgenic plants.
• To highlight recent improvements in optimizing protein yields.
• To focus on adjustable 'inside' and 'outside' factors influencing production.

Main Methods:

• Review of current literature on gene engineering for recombinant protein production in plants.
• Analysis of factors affecting mRNA sequence and structure ('inside' factors).
• Evaluation of host and production system/conditions ('outside' factors).

Main Results:

• Transgenic plants are a viable alternative to traditional expression systems.
• Gene engineering provides control over key parameters for yield optimization.
• Both intrinsic (mRNA) and extrinsic (host/environment) factors can be manipulated.

Conclusions:

• Transgenic plants represent a promising and optimizable platform for biopharmaceutical protein production.
• Strategic gene engineering of 'inside' and 'outside' factors is crucial for maximizing recombinant protein yields.
• Further advancements in understanding and controlling these factors will enhance plant-based biomanufacturing.