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Automated immunoassays for 25-hydroxyvitamin D (25-OHD): do plasticisers interfere?

G D Carter¹, J Jones¹, M Ketheeswaran¹

¹Department of Clinical Biochemistry, Department Imperial College Healthcare NHS Trust, Charing Cross Hospital, Fulham Palace Road, London W6 8RF, UK.

The Journal of Steroid Biochemistry and Molecular Biology

|December 3, 2014

Summary

A contaminant leached from plastic blood collection bags did not interfere with automated immunoassays for 25-hydroxyvitamin D (25-OHD). This finding is crucial for accurate vitamin D testing quality assessment.

Keywords:

25-Hydroxyvitamin D DEQAS Immunoassays Plasticisers

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Area of Science:

Clinical Chemistry
Analytical Chemistry
Laboratory Quality Assurance

Background:

The Vitamin D External Quality Assessment Scheme (DEQAS) monitors vitamin D metabolite testing quality.
A contaminant was identified in serum samples from plastic bags, potentially affecting 25-hydroxyvitamin D (25-OHD) measurements.
Concerns arose regarding interference in automated non-extraction assays for 25-OHD.

Purpose of the Study:

To investigate whether a contaminant leached from plastic blood collection bags interferes with automated immunoassays for 25-OHD.
To compare 25-OHD results from blood collected in plastic bags versus glass tubes using specific automated assays.

Main Methods:

Serum samples were collected simultaneously into plain glass tubes and plastic bags.
Results from three different automated non-extraction immunoassays for 25-OHD were compared between the two collection methods.
Statistical analysis was performed to determine significant differences in 25-OHD measurements.

Main Results:

No significant difference was observed in serum 25-OHD results between blood collected in plastic bags and glass tubes.
The contaminant leached from plastic bags did not interfere with the performance of the three automated immunoassays evaluated.
This suggests the contaminant does not impact the accuracy of these specific 25-OHD testing methods.

Conclusions:

The contaminant identified in serum from plastic blood collection bags does not interfere with the automated immunoassays for 25-OHD.
This finding reassures the reliability of these automated methods in quality assessment schemes like DEQAS.
Accurate vitamin D metabolite testing is essential for clinical diagnosis and patient management.