Related Experiment Video

Updated: Apr 20, 2026

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line
09:14

Isolation and Quantification of Epstein-Barr Virus from the P3HR1 Cell Line

Published on: September 28, 2022

4.9K

Epstein-Barr virus miR-BART20-5p regulates cell proliferation and apoptosis by targeting BAD

Hyoji Kim1, Hoyun Choi1, Suk Kyeong Lee1

  • 1Department of Medical Lifescience, College of Medicine, The Catholic University of Korea, 222 Banpo-daero, Seocho-gu, Seoul 137-701, South Korea.

Cancer Letters
|December 3, 2014
PubMed

Insights

Epstein-Barr virus BART miRNAs are common in tumors. This study identifies miR-BART20-5p as a key player, showing it targets BAD to promote cancer growth and chemoresistance in EBV-associated gastric carcinoma.

Area of Science:

  • Molecular Biology
  • Virology
  • Oncology

Background:

  • Epstein-Barr virus (EBV) BamHI A rightward transcript (BART) microRNAs (miRNAs) are frequently detected in EBV-associated malignancies.
  • The specific functions of most BART miRNAs remain largely uncharacterized.

Purpose of the Study:

  • To investigate the role of BART miRNAs in EBV-associated gastric carcinoma.
  • To determine if BART miRNAs regulate the expression of Bcl-2-associated death promoter (BAD).

Main Methods:

  • Bioinformatic analysis to identify potential BART miRNA targets of BAD.
  • Luciferase reporter assays to confirm direct targeting of BAD by miR-BART20-5p.
  • Transfection experiments with miR-BART20-5p and its inhibitor to assess effects on BAD expression, apoptosis, cell proliferation, and chemoresistance.

Main Results:

  • Bioinformatics predicted five BART miRNAs could target BAD; miR-BART20-5p was the only one to reduce BAD expression upon transfection.
  • Luciferase assays confirmed direct interaction between miR-BART20-5p and the 3'-UTR of BAD.
  • miR-BART20-5p significantly decreased BAD mRNA and protein levels, reduced apoptosis, enhanced cell proliferation, and increased resistance to 5-fluorouracil and docetaxel.

Conclusions:

  • miR-BART20-5p directly targets BAD in EBV-infected cells.
  • This interaction contributes to the tumorigenesis of EBV-associated gastric carcinoma by promoting cell survival and chemoresistance.
Keywords:
ApoptosisBADBART miRNACell proliferationEpstein–Barr virus

More Related Videos

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines06:38

Establishment of Epstein-Barr Virus Growth-transformed Lymphoblastoid Cell Lines

Published on: November 8, 2011

41.5K
In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells10:26

In Vitro Differentiation Model of Human Normal Memory B Cells to Long-lived Plasma Cells

Published on: January 20, 2019

13.4K

Related Concept Videos

Abnormal Proliferation02:23

Abnormal Proliferation

Under normal conditions, most adult cells remain in a non-proliferative state unless stimulated by internal or external factors to replace lost cells. Abnormal cell proliferation is a condition in which the cell's growth exceeds and is uncoordinated with normal cells. In such situations, cell division persists in the same excessive manner even after cessation of the stimuli, leading to persistent tumors. The tumor arises from the damaged cells that replicate to pass the damage to the...
5.5K
Negative Regulator Molecules01:23

Negative Regulator Molecules

Positive regulators allow a cell to advance through cell cycle checkpoints. Negative regulators have an equally important role as they terminate a cell’s progression through the cell cycle—or pause it—until the cell meets specific criteria.
39.2K
The Intrinsic Apoptotic Pathway01:31

The Intrinsic Apoptotic Pathway

Internal cellular stress, such as cellular injury or hypoxia, triggers intrinsic apoptosis. The B-cell lymphoma 2 (Bcl-2) family of proteins are the primary regulators of the intrinsic apoptotic pathway. For example, during DNA damage, checkpoint proteins, such as Ataxia Telangiectasia Mutated (ATM protein) and Checkpoints Factor-2 (Chk2) proteins, are activated. These proteins phosphorylate p53 which further activates pro-apoptotic proteins, such as Bax, Bak, PUMA, and Noxa, and inhibits...
9.4K
mTOR Signaling and Cancer Progression03:03

mTOR Signaling and Cancer Progression

The mammalian target of rapamycin or mTOR protein was discovered in 1994 due to its direct interaction with rapamycin. The protein gets its name from a yeast homolog called TOR. The mTOR protein complex in mammalian cells plays a major role in balancing anabolic processes such as the synthesis of proteins, lipids, and nucleotides and catabolic processes, such as autophagy in response to environmental cues, such as availability of nutrients and growth factors.
The mTOR pathway or the...
5.1K
PI3K/mTOR/AKT Signaling Pathway01:22

PI3K/mTOR/AKT Signaling Pathway

The mammalian target of rapamycin  (mTOR) is a serine/threonine kinase that regulates growth, proliferation, and cell survival in response to hormones, growth factors, or nutrient availability. This kinase exists in two structurally and functionally distinct forms: mTOR complex 1  (mTORC1) and mTOR complex 2  (mTORC2). The first form (mTORC1) is composed of a rapamycin-sensitive Raptor and proline-rich Akt substrate, PRAS40. In contrast,  mTORC2 consists of a...
6.5K
Mitogens and the Cell Cycle02:38

Mitogens and the Cell Cycle

Mitogens and their receptors play a crucial role in controlling the progression of the cell cycle. However, the loss of mitogenic control over cell division leads to tumor formation. Therefore, mitogens and mitogen receptors play an important role in cancer research. For instance, the epidermal growth factor (EGF) - a type of mitogen and its transmembrane receptor (EGFR), decides the fate of the cell's proliferation. When EGF binds to EGFR, a member of the ErbB family of tyrosine kinase...
8.5K