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Injectable Supramolecular Polymer-Nanoparticle Hydrogels for Cell and Drug Delivery Applications
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Injectable silk-polyethylene glycol hydrogels.

Xiaoqin Wang1, Benjamin Partlow1, Jian Liu2

  • 1National Engineering Laboratory for Modern Silk, Soochow University, Suzhou 215123, China; Department of Biomedical Engineering, Tufts University, Medford, MA 02155, USA.

Acta Biomaterialia
|December 3, 2014
PubMed
Summary
This summary is machine-generated.

Injectable silk hydrogels were created by mixing silk with polyethylene glycol (PEG). These novel PEG-silk hydrogels offer tunable gelation, slow degradation, and potential for anti-fouling biomedical applications.

Keywords:
HydrogelInjectablePolyethylene glycolSilkStem cells

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Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Polymer Chemistry

Background:

  • Silk hydrogels are valuable for tissue repair but often require pre-formation.
  • Injectable hydrogels at high concentrations (>8%) are desirable for minimally invasive medical applications.
  • Achieving slow in vivo degradation is crucial for effective tissue regeneration.

Purpose of the Study:

  • To develop injectable silk hydrogels formed in situ using low-molecular-weight polyethylene glycol (PEG).
  • To characterize the gelation properties, structural changes, and mechanical properties of PEG-silk hydrogels.
  • To evaluate the biocompatibility and in vivo performance of these novel hydrogels for biomedical applications.

Main Methods:

  • Mixing silk with low-molecular-weight PEG (PEG300 and PEG400) to form hydrogels in situ.
  • Assessing gelation time, optical density, and rheological properties.
  • Analyzing structural changes using circular dichroism, FTIR, and X-ray diffraction.
  • Evaluating injectability through fine needles and cell attachment of human mesenchymal stem cells.
  • Subcutaneous injection in rats to assess degradation and tissue in-growth via ultrasound and histology.

Main Results:

  • Injectable silk-PEG hydrogels were successfully formed in situ, with gelation time dependent on PEG concentration and molecular weight.
  • Gelation involved a structural transition of silk from random coil to β-sheets.
  • Mechanical properties were comparable to sonication-induced hydrogels.
  • Initial cell attachment was hindered, but significant degradation and tissue in-growth were observed in vivo without inflammation.
  • PEG concentration influenced gelation kinetics, with PEG400 faster than PEG300.

Conclusions:

  • PEG-silk hydrogels offer tunable injectability and slow degradation for biomedical applications.
  • The observed anti-fouling properties suggest potential for anti-adhesion uses.
  • These hydrogels represent a promising platform for minimally invasive tissue repair and regenerative medicine.