Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Sub-diffractional infrared absorption of two-dimensional water.

Nature communications·2026
Same author

Rapid single-live-cell scanning <i>via</i> Fourier-transform infrared (FTIR) microscopy to elucidate microplastic-cell interactions.

MethodsX·2026
Same author

Fourier Transform Infrared Microspectroscopy as a Liquid Biopsy Tool to Detect Single Circulating Tumour Cells in the Blood of a Lung Cancer Patient.

Applied spectroscopy·2025
Same author

Study of the biomolecular composition of skeletal muscle fibres affected by different types of pathology using by FTIR microspectroscopy.

Spectrochimica acta. Part A, Molecular and biomolecular spectroscopy·2025
Same author

Identification of tauopathy-associated lipid signatures in Alzheimer's disease mouse brain using label-free chemical imaging.

Communications biology·2024
Same author

Synchrotron nano-FTIR spectroscopy for probing anticancer drugs at subcellular scale.

Scientific reports·2024

Related Experiment Video

Updated: Apr 20, 2026

Author Spotlight: Advanced Techniques for Characterizing Tissue Mineralization in Bone Regeneration Research
07:29

Author Spotlight: Advanced Techniques for Characterizing Tissue Mineralization in Bone Regeneration Research

Published on: September 27, 2024

1.4K

Tracking calcification in tissue-engineered bone using synchrotron micro-FTIR and SEM.

Anthony J Deegan1, Gianfelice Cinque, Katia Wehbe

  • 1Institute for Science and Technology in Medicine, School of Medicine, Keele University, Stoke-on-Trent, ST4 7QB, UK.

Analytical and Bioanalytical Chemistry
|December 3, 2014
PubMed
Summary

Bone cell aggregate cultures enhance mineralization. Larger aggregates mineralize faster, with size influencing calcification patterns over time, as revealed by advanced imaging techniques.

More Related Videos

Hybrid &#181;CT-FMT imaging and image analysis
13:45

Hybrid µCT-FMT imaging and image analysis

Published on: June 4, 2015

13.8K
Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts
13:16

Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts

Published on: December 22, 2015

12.0K

Related Experiment Videos

Last Updated: Apr 20, 2026

Author Spotlight: Advanced Techniques for Characterizing Tissue Mineralization in Bone Regeneration Research
07:29

Author Spotlight: Advanced Techniques for Characterizing Tissue Mineralization in Bone Regeneration Research

Published on: September 27, 2024

1.4K
Hybrid &#181;CT-FMT imaging and image analysis
13:45

Hybrid µCT-FMT imaging and image analysis

Published on: June 4, 2015

13.8K
Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts
13:16

Computed Tomography and Optical Imaging of Osteogenesis-angiogenesis Coupling to Assess Integration of Cranial Bone Autografts and Allografts

Published on: December 22, 2015

12.0K

Area of Science:

  • Biomaterials Science
  • Tissue Engineering
  • Biophysics

Background:

  • Bone tissue engineering utilizes aggregate/micromass cultures to mimic in vivo bone formation.
  • Existing methods like histochemistry and RT-PCR lack simultaneous spatial and temporal resolution for proliferation and mineralization.
  • Synchrotron-based Fourier transform infrared microspectroscopy (micro-FTIR) provides high-resolution molecular-level insights.

Purpose of the Study:

  • To investigate the impact of culture duration and aggregate size on calcification dynamics and spatial distribution in engineered bone.
  • To compare the efficacy of micro-FTIR with SEM/EDX for tracking mineralization.

Main Methods:

  • Engineered bone aggregates (small/large) from a murine cell line were cultured on treated substrates.
  • Synchrotron-based Fourier transform infrared microspectroscopy (micro-FTIR) was used for molecular-level mapping.
  • Scanning electron microscopy/energy-dispersive X-ray spectroscopy (SEM/EDX) was employed for mineral content analysis.

Main Results:

  • Bone cell aggregate cultures significantly increased mineralization levels in short periods.
  • Larger aggregates exhibited faster mineralization rates compared to smaller ones.
  • Micro-FTIR mapping revealed distinct mineralization patterns: peripheral initiation in large aggregates and central initiation followed by peripheral deposition in small aggregates over time.
  • SEM/EDX results correlated well with micro-FTIR for total mineral content.

Conclusions:

  • Aggregate size critically influences mineralization rates and spatial distribution in engineered bone.
  • Synchrotron-based micro-FTIR is a powerful tool for accurately tracking mineralization processes in engineered bone.
  • This study enhances understanding of bone formation mechanisms in tissue engineering contexts.