Related Experiment Video

Updated: Apr 20, 2026

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation
07:14

A Flow Cytometry-based Assay for Measuring Mitochondrial Membrane Potential in Cardiac Myocytes After Hypoxia/Reoxygenation

Published on: July 13, 2018

15.5K

Angiotensin II induces mitochondrial oxidative stress and mtDNA damage in osteoblasts by inhibiting

Yong Li, Guangsi Shen, Chen Yu

    Biochemical and Biophysical Research Communications
    |December 3, 2014
    PubMed

    Insights

    Angiotensin II causes bone dysfunction by increasing mitochondrial oxidative stress and DNA damage in osteoblasts. Targeting the SIRT1-FoxO3a-MnSOD pathway may protect against this bone loss.

    Area of Science:

    • Biochemistry
    • Cell Biology
    • Bone Metabolism

    Background:

    • Angiotensin II is linked to osteoporosis, with some antihypertensive drugs showing protective effects.
    • The precise mechanisms of angiotensin II-induced bone dysfunction remain unclear.

    Purpose of the Study:

    • To elucidate the molecular mechanisms underlying angiotensin II-induced osteoblast dysfunction.
    • To investigate the role of mitochondrial oxidative stress and specific signaling pathways.

    Main Methods:

    • Osteoblast cell culture treated with angiotensin II.
    • Analysis of mitochondrial oxidative stress and DNA damage markers.
    • Western blotting and RT-PCR for protein and RNA expression (SIRT1, FoxO3a, catalase, MnSOD).
    • Treatment with SIRT1 activator (SRT3025) and MnSOD mimetic (MnTBAP).

    Main Results:

    • Angiotensin II significantly increased mitochondrial oxidative stress and mitochondrial DNA (mtDNA) damage in osteoblasts.
    • Decreased expression of mitochondrial catalase and manganese superoxide dismutase (MnSOD).
    • Inhibition of SIRT1 led to FoxO3a hyperacetylation, reducing catalase and MnSOD expression.
    • SRT3025 and MnTBAP treatments ameliorated oxidative stress and mtDNA damage.

    Conclusions:

    • A novel SIRT1–FoxO3a–MnSOD signaling axis mediates angiotensin II-induced mitochondrial damage in osteoblasts.
    • This pathway represents a potential therapeutic target for preventing angiotensin II-related bone disorders.

    More Related Videos

    Author Spotlight: New Insights into PBMC Mitochondrial Responses Using Fluorespirometry07:18

    Author Spotlight: New Insights into PBMC Mitochondrial Responses Using Fluorespirometry

    Published on: May 24, 2024

    1.9K
    Analyzing Oxygen Consumption Rate in Primary Cultured Mouse Neonatal Cardiomyocytes Using an Extracellular Flux Analyzer11:26

    Analyzing Oxygen Consumption Rate in Primary Cultured Mouse Neonatal Cardiomyocytes Using an Extracellular Flux Analyzer

    Published on: February 13, 2019

    9.3K

    Related Concept Videos

    Electron Transport Chain: Complex I and II01:46

    Electron Transport Chain: Complex I and II

    The mitochondrial electron transport chain (ETC) is the main energy generation system in the eukaryotic cells. However, mitochondria also produce cytotoxic reactive oxygen species (ROS) due to the large electron flow during oxidative phosphorylation. While Complex I is one of the primary sources of superoxide radicals, ROS production by Complex II is uncommon and may only be observed in cancer cells with mutated complexes.
    ROS generation is regulated and maintained at moderate levels necessary...
    19.8K
    Mitochondria01:37

    Mitochondria

    Mitochondria are eukaryotic cellular organelles that are known to produce energy through a process called oxidative phosphorylation. Besides their primary function, mitochondria are involved in various cellular processes, including cell growth, differentiation, signaling, metabolism, and senescence. Age-related changes cause a decline in mitochondrial quality and integrity due to increased mitochondrial mutations and oxidative damage. Thus, aging can severely impact mitochondrial functions,...
    21.7K
    Mitochondrial Membranes01:45

    Mitochondrial Membranes

    A single mitochondrion is a bean-shaped organelle enclosed by a double-membrane system. The outer membrane of mitochondria is smooth and contains many porins - the integral membrane transporters. Porins enable free diffusion of ions and small uncharged molecules through the outer mitochondrial membrane but limit the transport of molecules larger than 5000 Daltons. Further, the outer mitochondrial membrane forms a unique structure called membrane contact sites with other subcellular organelles,...
    18.1K
    Translocation of Proteins into the Mitochondria01:19

    Translocation of Proteins into the Mitochondria

    Mitochondrial precursors are translocated to the internal subcompartments via independent mechanisms involving distinct protein machineries called translocases.
    Sorting of outer membrane proteins:
    Mitochondrial outer membrane proteins are of two types: the transmembrane, beta-barrel porins, and the membrane-anchored, alpha-helical proteins. Beta-barrel porin precursors are translocated by the TOM complex and inserted into the outer mitochondrial membrane by the SAM complex. In contrast,...
    13.8K
    The Electron Transport Chain01:30

    The Electron Transport Chain

    The electron transport chain or oxidative phosphorylation is an exothermic process in which free energy released during electron transfer reactions is coupled to ATP synthesis. This process is a significant source of energy in aerobic cells, and therefore inhibitors of the electron transport chain can be detrimental to the cell's metabolic processes.
    Inhibitors of the electron transport chain
    Rotenone, a widely used pesticide, prevents electron transfer from Fe-S cluster to ubiquinone or Q...
    21.9K
    Cellular Injury I: Introduction01:00

    Cellular Injury I: Introduction

    Cellular injury occurs when a cell cannot maintain homeostasis or adapt to stressors such as hypoxia, toxins, or trauma. Depending on severity and duration, injury may be reversible, allowing recovery, or irreversible, leading to cell death.General Mechanisms of Cell InjuryAlthough causes vary, most cellular injuries arise from a few key mechanisms that disrupt essential functions and often amplify one another. Cell survival depends on the extent and balance of these disturbances.ATP depletion...
    6