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A new method for polyhydroxyalkanoate (PHA) accumulating bacteria selection under physical selective pressure.

Zhiqiang Chen¹, Zirui Guo¹, Qinxue Wen¹

¹State Key Laboratory of Urban Water Resource and Environment, Harbin Institute of Technology (SKLUWRE, HIT), Harbin 150090, China.

International Journal of Biological Macromolecules

|December 3, 2014

Summary

This summary is machine-generated.

A novel aerobic dynamic discharge (ADD) process enhances polyhydroxyalkanoates (PHA) production by selecting PHA-accumulating bacteria. This method offers superior PHA content and faster selection times compared to traditional aerobic dynamic feeding (ADF).

Keywords:

Aerobic dynamic discharge (ADD)Aerobic dynamic feeding (ADF)Mixed microbial culture (MMC)

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Area of Science:

Microbiology
Biotechnology
Environmental Engineering

Background:

Microbial mixed cultures (MMCs) are utilized for polyhydroxyalkanoates (PHA) production.
Optimizing PHA accumulation requires effective selection strategies for PHA-accumulating bacteria.
Conventional aerobic dynamic feeding (ADF) has limitations in maximizing PHA yield and selection efficiency.

Purpose of the Study:

To propose and evaluate a new aerobic dynamic discharge (ADD) process for PHA production.
To compare the efficacy of the ADD process against the conventional ADF mode.
To investigate the impact of physical selective pressure on PHA accumulation in MMCs.

Main Methods:

Implementation of a sequencing batch reactor operating in ADD mode.
Comparison of PHA production under ADD and ADF modes.
Analysis of bacterial culture selection under different dynamic discharge strategies.

Main Results:

The ADD mode demonstrated superior PHA-producing potential compared to the ADF mode.
Cultures selected under ADD exhibited higher maximum PHA content (74.16%) and yield (0.72 C mol PHA/C mol Ac).
The ADD process achieved faster selection of PHA-accumulating bacteria.

Conclusions:

The ADD process offers a promising and efficient method for the rapid selection of strong PHA-accumulating bacterial cultures.
Altered physical selective pressure in the ADD mode significantly enhances PHA accumulation.
ADD represents an advancement over ADF for optimizing microbial PHA production.