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ELISA for detection of antibodies to a type D retrovirus, SRV-W
K T Schultz1, C Thomas, K Toohey
1Department of Pathobiological Sciences, School of Veterinary Medicine, University of Wisconsin, Madison.
Viral Immunology
|January 1, 1989
Abstract:
An ELISA was developed to detect antibodies to a Type D retrovirus, SRV-W. The interpretation of the ELISA results were based on: 1) Comparisons of known antibody positive (to a closely related type D retrovirus) and negative serum samples, 2) the ability of ELISA reactivity to be absorbed with a Type D virus but not a mock virus preparation, and 3) analysis by a Western Blot assay as an alterative way to identify antibody to the Type D retrovirus.