Related Experiment Video
Updated: Apr 20, 2026

Ion Exchange Chromatography IEX Coupled to Multi-angle Light Scattering MALS for Protein Separation and Characterization
Published on: April 5, 2019
Ion exchange membranes as novel passive sampling material for organic ions: application for the determination of
Luise Oemisch1, Kai-Uwe Goss2, Satoshi Endo1
1Helmholtz Centre for Environmental Research UFZ, Permoserstr. 15, D-04318 Leipzig, Germany.
Abstract:
Many studies in pharmacology, toxicology and environmental science require a method for determining the freely dissolved concentration of a target substance. A recently developed tool for this purpose is equilibrium passive sampling with polymeric materials. However, this method has rarely been applied to ionic organic substances, primarily due to limited availability of convenient sorption materials. This study introduces ion exchange membranes (IEMs) as a novel passive sampling material for organic ions. The partitioning of 4-ethylbenzene-1-sulfonate, 2,4-dichlorophenoxyacetic acid and pentachlorophenol to one anion exchange membrane (FAS) and of difenzoquat, nicotine and verapamil to one cation exchange membrane (FKS) was investigated. All test substances exhibited a sufficiently high affinity for the respective IEM with logarithmic IEM-water partition coefficients >2.3. Sorption equilibrium was established quickly, within several hours for the FAS membrane and within 1-3 days for the FKS membrane. For permanently charged substances the partitioning to the IEMs was independent of pH, but was influenced by the salt composition of the test solution. For all test substances sorption to IEM was dependent on the substance concentration. Bovine serum albumin-water partition coefficients determined by passive sampling with IEMs agree well with those determined by the conventional dialysis method. The results of this study indicate that IEMs exhibit the potential to measure freely dissolved concentrations of organic ions in a simple and time-saving manner.
More Related Videos
Related Concept Videos
Ion Exchange
Ion-Exchange Chromatography
Potentiometry: Membrane Electrodes
Dialysis
Extraction: Advanced Methods
Analyte Adsorption and Distribution

