Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Oxygen Transport in the Blood01:27

Oxygen Transport in the Blood

8.9K
Hemoglobin (Hb) is a crucial molecule in the human body, consisting of four polypeptide chains, each bound to an iron-containing heme group. This unique structure enables hemoglobin to bind to oxygen, with each molecule capable of combining with four molecules of oxygen, leading to rapid and reversible oxygen loading. When fully loaded with oxygen, it is called oxyhemoglobin, while hemoglobin that has released oxygen is called reduced hemoglobin or deoxyhemoglobin. As hemoglobin binds oxygen,...
8.9K
Osteoclasts in Bone Remodeling01:31

Osteoclasts in Bone Remodeling

4.9K
Osteoclasts are cells responsible for bone resorption and remodeling. They originate from hematopoietic progenitor cells present in the bone marrow. Numerous progenitor cells fuse to form multinucleated cells, each with 10-20 nuclei. A single osteoclast has a diameter of 150 to 200 µM. These cells have ruffled borders that break down the underlying bone tissue and release minerals such as calcium into the blood in bone resorption. Osteoclasts cling to bones with their ruffled edges during...
4.9K
Treatment for Pulmonary Arterial Hypertension: Oxygen Therapy for Respiratory Failure01:16

Treatment for Pulmonary Arterial Hypertension: Oxygen Therapy for Respiratory Failure

811
Oxygen therapy has emerged as a significant tool in enhancing the quality of life for patients suffering from pulmonary arterial hypertension (PAH). While this therapy has principally been studied on patients with significant hypoxemia, this therapeutic approach helps prevent potential organ damage and can be administered in the comfort of one's home.
Oxygen therapy is vital in increasing and maintaining blood oxygen levels in PAH patients. As a result, it aids in reducing fatigue,...
811
Bone Remodeling01:40

Bone Remodeling

41.3K
Bone remodeling is a continuous and balanced process of bone resorption by osteoclasts and bone formation by osteoblasts. In adults, it helps maintain bone mass and calcium homeostasis. While mechanical stress can stimulate turnover as part of the normal maintenance and reparative process, several hormones also regulate bone remodeling.
41.3K
Bone Formation by Endochondral Ossification01:24

Bone Formation by Endochondral Ossification

16.1K
Bone formation, or ossification, begins around the sixth to seventh week of embryonic development. Most bones develop from a cartilaginous template through the process of endochondral ossification. Cartilage formation begins when clusters of mesenchymal cells differentiate into chondrocytes. These chondrocytes proliferate rapidly and secrete an extracellular matrix that becomes encased in a membrane called the perichondrium. The resulting cartilage model provides a template that resembles the...
16.1K
Hormones and Bone Tissue01:17

Hormones and Bone Tissue

4.3K
The endocrine system produces and secretes hormones, which interact with the skeletal system. These hormones control bone growth, maintain bone once it is formed, and remodel it.
Hormones That Influence Osteoblasts and/or Maintain the Matrix
Several hormones are necessary for controlling bone growth and maintaining the bone matrix. The pituitary gland secretes growth hormone (GH), which, as its name implies, controls bone growth. This happens in several ways: first, it triggers chondrocyte...
4.3K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

d-limonene suppresses RANKL-induced osteoclast differentiation and promotes osteoblast activity in vitro.

Bioscience, biotechnology, and biochemistry·2024
Same author

Cellular Pre-Adaptation to the High O<sub>2</sub> Concentration Used in Standard Cell Culture Confers Resistance to Subsequent H<sub>2</sub>O<sub>2</sub>-Induced Cell Death.

Antioxidants (Basel, Switzerland)·2024
Same author

Aural health awareness and incident prevention in UK scuba divers.

Diving and hyperbaric medicine·2022
Same author

Flying after diving: a questionnaire-based evaluation of pre-flight diving behaviour in a recreational diving cohort.

Diving and hyperbaric medicine·2021
Same author

Diving and mental health: the potential benefits and risks from a survey of recreational scuba divers.

Diving and hyperbaric medicine·2019
Same author

Altered cellular redox homeostasis and redox responses under standard oxygen cell culture conditions versus physioxia.

Free radical biology & medicine·2018

Related Experiment Video

Updated: Apr 20, 2026

Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration
06:05

Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration

Published on: July 14, 2023

1.8K

Hyperbaric oxygen therapy accelerates osteoblast differentiation and promotes bone formation.

Hadil Al Hadi1, Gary R Smerdon2, Simon W Fox1

  • 1School of Biomedical and Healthcare Sciences, Plymouth University, Plymouth, UK.

Journal of Dentistry
|December 3, 2014
PubMed
Summary

Hyperbaric oxygen therapy (HBO) accelerates osteoblast differentiation and bone formation. This study shows HBO enhances bone nodule formation, particularly in hypoxic conditions, suggesting a direct benefit for osteonecrotic bone loss.

Keywords:
Bone formationHyperbaric oxygen therapyOsteonecrosis of the jaw

More Related Videos

Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics
09:43

Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics

Published on: March 1, 2022

3.9K
Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect
09:35

Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect

Published on: September 11, 2015

10.2K

Related Experiment Videos

Last Updated: Apr 20, 2026

Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration
06:05

Author Spotlight: Enhancing Bone Regeneration with Vascularized Artificial Cartilage Integration

Published on: July 14, 2023

1.8K
Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics
09:43

Using Real-Time Cell Metabolic Flux Analyzer to Monitor Osteoblast Bioenergetics

Published on: March 1, 2022

3.9K
Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect
09:35

Distinctive Capillary Action by Micro-channels in Bone-like Templates can Enhance Recruitment of Cells for Restoration of Large Bony Defect

Published on: September 11, 2015

10.2K

Area of Science:

  • Biomedical Engineering
  • Regenerative Medicine
  • Oral and Maxillofacial Surgery

Background:

  • Osteonecrosis of the jaw (ONJ) is a challenging condition often treated with hyperbaric oxygen therapy (HBO).
  • The precise mechanisms by which HBO influences osteoblast activity in ONJ remain incompletely understood.
  • This study investigates HBO's direct impact on osteoblast differentiation and bone mineralization.

Purpose of the Study:

  • To evaluate the effects of HBO on osteoblast differentiation and bone nodule formation.
  • To compare the efficacy of HBO with elevated pressure or oxygen alone.
  • To elucidate HBO's role in promoting bone repair in osteonecrosis.

Main Methods:

  • Saos-2 human osteoblast cells were cultured under normoxic or hypoxic conditions.
  • Cells were exposed to HBO (2.4 ATA, 97.9% O2), elevated pressure (2.4 ATA), or elevated oxygen (1 ATA) daily.
  • Osteoblast differentiation and bone formation were assessed via alkaline phosphatase activity, calcein incorporation, and gene expression analysis (COL1, Runx-2).

Main Results:

  • Daily HBO treatment significantly accelerated osteoblast differentiation, evidenced by increased alkaline phosphatase activity and collagen type I/Runx-2 mRNA expression.
  • HBO augmented bone nodule formation, especially under hypoxic conditions.
  • The effects of HBO on osteoblast differentiation markers were more pronounced than those of elevated pressure or oxygen alone.

Conclusions:

  • Hyperbaric oxygen therapy demonstrably accelerates osteoblast differentiation and enhances bone formation.
  • HBO may improve surgical outcomes in osteonecrosis by directly promoting osteoblast activity and increasing bone mass for reconstruction.
  • These findings contribute to understanding HBO's reparative actions in osteonecrotic bone loss.