Constituent analysis and quality control of Lamiophlomis rotata by LC-TOF/MS and HPLC-UV

In the present study, chemical profile analysis for Lamiophlomis rotata, a classic Tibetan folk medicine, was illustrated by an ultra-high performance liquid chromatography quadrupole time-of-flight mass spectrometry (LC-TOF/MS) method, which provided evidence for the certain identification of the main constituents, including iridoids, flavonoids and phenylethanoid glycosides. Among these compounds, nine of them were regarded as marker compounds for the quantitative evaluation of L. rotata, using a simple and reliable method by HPLC with ultraviolet, in combination of chromatographic fingerprint analysis. Separation was achieved on a Waters SunFire C18 analytical column with linear gradient elution of acetonitrile and 0.1% formic acid aqueous solution. The validated method was successfully applied to evaluate twelve batches of L. rotata. Assay results showed that nine compounds did not vary significantly from the aerial parts and the whole plant for each batch, and was consistent with the fingerprint analysis, which confirmed the medicine parts alteration in Chinese Pharmacopoeia from the perspective of chemical components.