Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

8.6K
Wnt is a zygotic effect gene that is expressed during very early embryonic development. It regulates various processes in animals starting from early development through the adult stage, such as organogenesis in the embryo and maintenance of neuronal and blood stem cells. Wnt proteins can induce a wide variety of intracellular pathways depending upon the specific abilities of different Wnt ligands to form a complex with shared and cognate receptors in the presence of different co-receptors. The...
8.6K
Non-Canonical Wnt Signaling Pathways01:41

Non-Canonical Wnt Signaling Pathways

1.9K
1.9K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

11.0K
The gene encoding the main signaling molecules of the Wnt signaling pathways (the Wnt proteins) was discovered almost four decades ago by Nüsslein-Volhard and Wieschaus. They identified and originally named the gene "wingless" (wg) after a phenotype discovered during their landmark genetic screen in Drosophila for body pattern defects. At around the same time, another researcher named Harold Varmus found that a murine tumor virus activates the mammalian wg homolog, Int-1, which...
11.0K
Canonical Wnt Signaling Pathway02:54

Canonical Wnt Signaling Pathway

2.7K
2.7K
Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal01:22

Role of Ephrin-Eph Signalling in Intestinal Stem Cell Renewal

2.8K
Erythropoietin-producing hepatocellular carcinoma receptor (Eph) and its ligand, Eph receptor-interacting protein (Ephrin) were first discovered in the human carcinoma cell line, hence the name. Ephrin-Eph interaction guides cells to reach their appropriate location in adult tissues. They also play an essential role in the immune system by helping in immune cell migration, adhesion, and activation. Based on their structure and function, Eph is divided into two classes — EphA and EphB.
2.8K
Role Of Notch Signalling In Intestinal Stem Cell Renewal01:12

Role Of Notch Signalling In Intestinal Stem Cell Renewal

2.6K
Notch signaling was first discovered in Drosophila melanogaster, where it is involved in cell lineage differentiation. Notch signaling regulates the maintenance and differentiation of intestinal stem cells or ISCs by controlling the expression of atonal homolog 1 or Atoh1. Atoh1 directs cells to differentiate into secretory cells.
Direct cell-to-cell contact is needed for the activation of Notch signaling. The signal is initiated when a notch ligand binds to a receptor on an adjacent cell, also...
2.6K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Mechanoreceptor plexin D1 regulates lymphatic valve morphogenesis and lymphedema pathogenesis.

The Journal of clinical investigation·2026
Same author

GABA <sub>A</sub> receptor gating imaged on the millisecond timescale.

bioRxiv : the preprint server for biology·2025
Same author

Protein Target Highlights in CASP16: Insights From the Structure Providers.

Proteins·2025
Same author

Mechanism of NACHO-mediated assembly of pentameric ligand-gated ion channels.

bioRxiv : the preprint server for biology·2024
Same author

Structural insights into Frizzled3 through nanobody modulators.

Nature communications·2024
Same author

Immunogenicity of Abdala COVID-19 vaccine in Vietnamese people after primary and booster vaccinations: A prospective observational study in Vietnam.

International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases·2024

Related Experiment Video

Updated: Apr 20, 2026

Modeling Paracrine Noncanonical Wnt Signaling In Vitro
11:14

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

2.0K

Extracellular modulators of Wnt signalling.

Tomas Malinauskas1, E Yvonne Jones2

  • 1Cold Spring Harbor Laboratory, W. M. Keck Structural Biology Laboratory, Cold Spring Harbor, NY 11724, USA.

Current Opinion in Structural Biology
|December 3, 2014
PubMed
Summary

Wnt morphogens regulate development and tissue repair. Recent structural studies reveal how cell surface interactions control Wnt signaling, offering insights into developmental biology and disease.

More Related Videos

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients
08:10

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Published on: December 14, 2015

11.9K
The Soft Agar Colony Formation Assay
08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

114.6K

Related Experiment Videos

Last Updated: Apr 20, 2026

Modeling Paracrine Noncanonical Wnt Signaling In Vitro
11:14

Modeling Paracrine Noncanonical Wnt Signaling In Vitro

Published on: December 10, 2021

2.0K
Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients
08:10

Using Confocal Analysis of Xenopus laevis to Investigate Modulators of Wnt and Shh Morphogen Gradients

Published on: December 14, 2015

11.9K
The Soft Agar Colony Formation Assay
08:01

The Soft Agar Colony Formation Assay

Published on: October 27, 2014

114.6K

Area of Science:

  • Molecular Biology
  • Developmental Biology
  • Biochemistry

Background:

  • Wnt morphogens are crucial signaling proteins involved in embryogenesis and adult tissue homeostasis.
  • Wnt signaling pathways are tightly regulated by various mechanisms, including posttranslational modifications and interactions with antagonists and receptors.

Purpose of the Study:

  • To review recent advancements in understanding Wnt signaling regulation at the cell surface.
  • To highlight key structural and biophysical studies that elucidate Wnt-receptor interactions and antagonist binding.

Main Methods:

  • Structure-guided biophysical studies
  • Crystallography
  • Cell-based assays

Main Results:

  • Detailed structures of Wnt complexes with Frizzled (CRD), LRP6, and antagonists like Dickkopf and Sclerostin have been determined.
  • Structures also include complexes with antagonists 5T4/WAIF1, WIF-1, and Frizzled-ubiquitin ligases ZNRF3/RNF43.
  • Insights into the regulation of Wnt receptor availability and signaling modulation by these complexes were gained.

Conclusions:

  • Recent structural and biophysical studies have significantly advanced the understanding of cell surface regulation of Wnt signaling.
  • Further research is needed to address remaining questions regarding the dynamic mechanisms controlling Wnt pathway activity.