Related Experiment Video
Updated: Apr 20, 2026
A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
Published on: February 28, 2015
A sensitive sequential 'on/off' SERS assay for heparin with wider detection window and higher reliability based on
Yi Zeng1, Jin-Ju Pei1, Li-Hua Wang1
1Key Laboratory of Analytical Chemistry for Biology and Medicine (Ministry of Education), College of Chemistry and Molecular Sciences, Wuhan University, Wuhan 430072, China.
Abstract:
A sequential 'on/off' dual mode SERS assay platform for heparin with wider detection window and higher reliability is constructed based on electrostatic forces, in which the highly protonated chitosan encapsulated p-Mercaptobenzoic acid coated Au@Ag core-shell nanoparticles undergo sequential aggregation/segregation upon the additive of heparin with a limit of detection of 43.74ng/mL (5.69U/mL) and a continuous concentration range of 50-800ng/mL (6.5-104U/mL), which are lower in sensitivity and wider in detection window than the most reported assay for heparin. Remarkably, the latter declined window over a range of 350-800ng/mL in contrast, which has not reported before, is extremely important in reliable and practical assay of heparin.
