Related Experiment Video
Updated: Apr 20, 2026

Using Fluorescent Proteins to Visualize and Quantitate Chlamydia Vacuole Growth Dynamics in Living Cells
Published on: October 13, 2015
Making connections: snapshots of chlamydial type III secretion systems in contact with host membranes
Maud Dumoux1, Andrea Nans2, Helen R Saibil2
1Institute of Structural and Molecular Biology, University College London & Birkbeck, Malet Street, London WC1E 7HX, UK.
Abstract:
Chlamydiae are obligate intracellular bacterial pathogens with an unusual biphasic lifecycle, which is underpinned by two bacterial forms of distinct structure and function. Bacterial entry and replication require a type III secretion system (T3SS), a widely conserved nanomachine responsible for the translocation of virulence effectors into host cells. Recent cell biology experiments supported by electron and cryo-electron tomography have provided fresh insights into Chlamydia-host interactions. In this review, we highlight some of the recent advances, particularly the in situ analysis of T3SSs in contact with host membranes during chlamydial entry and intracellular replication, and the role of the host rough endoplasmic reticulum (rER) at the recently described intracellular 'pathogen synapse'.
More Related Videos
Related Concept Videos
Bacterial Phylum Chlamydiae
Gram-negative Bacterial Protein Secretion Systems
Mechanism of Conjugation
Adherens Junctions
Adherens Junctions are Dynamic
Bacterial Translocation and Protein Secretion
CRISPR and crRNAs
The CRISPR-Cas system stores a copy of foreign DNA in the host genome and uses it to identify the foreign DNA upon reinfection. CRISPR-Cas has three different...

