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Updated: Dec 12, 2025

A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells

A Multimodal Imaging Framework to Advance Phenotyping of Living Label-free Breast Cancer Cells

Published on: August 22, 2025

Live-cell bioorthogonal Raman imaging.

Senlian Hong¹, Liang Lin¹, Min Xiao¹

¹Beijing National Laboratory for Molecular Sciences, Key Laboratory of Bioorganic Chemistry and Molecular Engineering of Ministry of Education, College of Chemistry and Molecular Engineering, Synthetic and Functional Biomolecules Center, and Peking-Tsinghua Center for Life Sciences, Peking University, Beijing 100871, China.

Current Opinion in Chemical Biology

|December 3, 2014

Summary

This summary is machine-generated.

Live-cell imaging is enhanced by bioorthogonal Raman imaging, which uses specialized small molecules for precise visualization without interfering with cellular processes. This review covers new methods and applications for this powerful live-cell imaging technique.

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Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Non-contact, Label-free Monitoring of Cells and Extracellular Matrix using Raman Spectroscopy

Published on: May 29, 2012

Area of Science:

Cellular imaging
Spectroscopy
Biochemistry

Background:

Live-cell microscopy requires high specificity, sensitivity, and minimal disruption of biomolecules.
Achieving these criteria simultaneously in cellular imaging remains a significant challenge.
Existing methods often struggle with signal overlap and cellular perturbation.

Purpose of the Study:

To review recent advancements in bioorthogonal Raman imaging for live-cell applications.
To highlight the methodological development of this technique.
To showcase proof-of-concept applications in cellular visualization.

Main Methods:

Utilizing small Raman reporters with non-overlapping signals.
Metabolic incorporation of Raman reporters into target biomolecules.
Development of bioorthogonal Raman imaging techniques for live-cell analysis.

Main Results:

Bioorthogonal Raman imaging enables visualization of biomolecules with high specificity.
Raman reporters are metabolically incorporated, allowing direct tracking.
The technique minimizes perturbation to cellular functions.
Successful proof-of-concept applications demonstrate its utility.

Conclusions:

Bioorthogonal Raman imaging offers a promising solution for challenging live-cell imaging.
Methodological innovations are expanding its capabilities.
This technique provides a powerful tool for studying cellular processes in real-time.