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Published on: May 24, 2024
Involvement of soil bacteria in ABO blood mistyping
Naoki Takada1, Chikahiro Mori2, Rie Takai2
1Science of Biological Resources, United Graduate School of Agricultural Sciences, Gifu University, Gifu 501-1193, Japan; Forensic Science Laboratory, Gifu Prefectural Police Headquarters, Gifu 500-8501, Japan.
Abstract:
The current study investigated whether ABO blood mistyping of human biological samples is induced by soil bacteria. A total of 380 bacterial strains were isolated from 50 discrete soil samples using human blood agar, and glycosidase activity evaluated for all strains using 4-nitropheny glycosides (4-nitrophenyl n-acetyl-α-D-galactosaminide, 4-nitrophenyl-α-D-galactopyranoside, 4-nitrophenyl-α-L-fucopyranoside) as substrates. Thirteen strains possessed α-galactosidase activity, and 16S rRNA sequence analysis revealed a close relatedness to the genus Bacillus. An indirect competitive enzyme-linked immunosorbent assay confirmed seven strains exhibited type B antigen degradation activity. These results demonstrated that 1.8% of the bacteria isolated from soil, were Bacillus sp., possessed galactosidase activity, and had the potential to cause ABO blood mistyping.
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