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Combining Analysis of DNA in a Crude Virion Extraction with the Analysis of RNA from Infected Leaves to Discover New Virus Genomes
Published on: July 27, 2018
Genome sequence of a virus isolate from tamarillo (Solanum betaceum) in Colombia: evidence for a new potyvirus
Pablo A Gutiérrez1, Juan F Alzate, Mauricio Marín Montoya
1Laboratorio de Microbiología Industrial, Facultad de Ciencias, Universidad Nacional de Colombia Sede Medellín, Calle 59A No 63-20, Medellín, Colombia, paguties@unal.edu.co.
Abstract:
Based on the results of a deep sequencing transcriptome study of tamarillo (Solanum betaceum), we report the genome sequence of a virus from this host plant. Since this probably represents a new member of the genus Potyvirus, the name tamarillo leaf malformation virus (TaLMV) has been proposed. Phylogenetic analysis reveals that TaLMV is the closest relative of Colombian datura virus (CDV), followed by three other potyviruses: tobacco etch virus, potato virus A and tobacco vein mottling virus. This is the first sequence of a potyvirus infecting Solanum betaceum containing the complete polyprotein coding region.
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