Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Microfluidic rapid isolation and electrochemical detection of S. pneumonia via aptamer-decorated surfaces.

Analytica chimica acta·2025
Same author

Preparation of interpenetrating networks from chitosan and poly(hydroxypropyl methacrylate) or p(hydroxyethyl methacrylate) for controlled release of doxorubicin and curcumin: Investigation of potential use in wound dressing.

International journal of biological macromolecules·2025
Same author

Enhanced SELEX Platforms for Aptamer Selection with Improved Characteristics: A Review.

Molecular biotechnology·2024
Same author

Quartz crystal microbalance-based aptasensor integrated with magnetic pre-concentration system for detection of Listeria monocytogenes in food samples.

Mikrochimica acta·2024
Same author

Fluorescent and electrochemical detection of nuclease activity associated with <i>Streptococcus pneumoniae</i> using specific oligonucleotide probes.

The Analyst·2024
Same author

Real-Time Biosensing Bacteria and Virus with Quartz Crystal Microbalance: Recent Advances, Opportunities, and Challenges.

Critical reviews in analytical chemistry·2023

Related Experiment Video

Updated: Apr 20, 2026

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System
06:58

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Published on: June 13, 2010

10.1K

Pathogen detection in complex samples by quartz crystal microbalance sensor coupled to aptamer functionalized

Veli C Ozalp1, Gulay Bayramoglu2, Zehra Erdem2

  • 1School of Medicine, Istanbul Kemerburgaz University, 34217 Istanbul, Turkey.

Analytica Chimica Acta
|December 4, 2014
PubMed
Summary

This study introduces a novel sensor for detecting Salmonella bacteria in food. It combines magnetic beads and a quartz crystal microbalance (QCM) for rapid, sensitive, and specific pathogen identification.

Keywords:
AptamersBiosensorsFood controlMagnetic beadsQuartz crystal microbalance

More Related Videos

Sample Preparation in Quartz Crystal Microbalance Measurements of Protein Adsorption and Polymer Mechanics
08:21

Sample Preparation in Quartz Crystal Microbalance Measurements of Protein Adsorption and Polymer Mechanics

Published on: January 22, 2020

14.3K
Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

5.7K

Related Experiment Videos

Last Updated: Apr 20, 2026

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System
06:58

Rapid Homogeneous Detection of Biological Assays Using Magnetic Modulation Biosensing System

Published on: June 13, 2010

10.1K
Sample Preparation in Quartz Crystal Microbalance Measurements of Protein Adsorption and Polymer Mechanics
08:21

Sample Preparation in Quartz Crystal Microbalance Measurements of Protein Adsorption and Polymer Mechanics

Published on: January 22, 2020

14.3K
Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions
08:31

Biosensor-based High Throughput Biopanning and Bioinformatics Analysis Strategy for the Global Validation of Drug-protein Interactions

Published on: December 1, 2020

5.7K

Area of Science:

  • Biosensor technology
  • Food safety analysis
  • Microbial detection

Background:

  • Detecting bacterial pathogens like Salmonella in food is crucial for public health.
  • Existing sensor methods often struggle with sensitivity, specificity, and robustness in complex food matrices.
  • Integrating pre-purification systems with biosensors offers a promising approach to overcome these limitations.

Purpose of the Study:

  • To develop a sensitive and specific method for detecting Salmonella enterica serovar typhimurium in food samples.
  • To integrate an aptamer-based magnetic separation system with a quartz crystal microbalance (QCM) sensor.
  • To establish a rapid, high-throughput strategy for pathogen detection in food.

Main Methods:

  • Developed an aptamer-based magnetic bead system for rapid enrichment of Salmonella cells.
  • Integrated the magnetic separation system with an aptamer-functionalized QCM sensor.
  • Tested the integrated system for Salmonella detection in a model food sample (milk).
  • Utilized NaOH solution for regenerating the QCM sensor surface for reusability.

Main Results:

  • Achieved efficient capture of Salmonella cells within 10 minutes using the magnetic separation system.
  • Demonstrated specific detection of Salmonella cells at concentrations as low as 100 CFU mL(-1) in milk.
  • Showcased the reusability of the aptamer-sensor through NaOH-based regeneration.

Conclusions:

  • The integrated aptamer-magnetic bead-QCM system provides a novel, sensitive, and specific platform for Salmonella detection in food.
  • This approach offers a rapid and robust solution for pathogen monitoring in food safety applications.
  • The sensor's reusability enhances its practicality and cost-effectiveness for routine analysis.