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Related Concept Videos

Enzyme-Linked Immunosorbent Assay01:33

Enzyme-Linked Immunosorbent Assay

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In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen.  Enzyme-substrate reaction allows the antigen to be visualized or...
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Related Experiment Video

Updated: Jun 16, 2025

A Method for Selecting Structure-switching Aptamers Applied to a Colorimetric Gold Nanoparticle Assay
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Enhanced SELEX Platforms for Aptamer Selection with Improved Characteristics: A Review.

Reza Didarian1, Hatice K Ozbek2, Veli C Ozalp3

  • 1Department of Biomedical Engineering, Faculty of Engineering and Natural Sciences, Ankara Yıldırım Beyazıt University, Ayvalı Mh. Takdir Cad.150 Sk. No:5, Etlik-Keçiören, Ankara, 06010, Türkiye.

Molecular Biotechnology
|August 16, 2024
PubMed
Summary

This review explores advanced aptamer selection (SELEX) techniques and modifications to improve their stability and targeting. These innovations enhance aptamer applications in diagnostics, therapeutics, and nanotechnology.

Keywords:
AptamersChemical modificationsNext-generation SELEX platformsPost-aptamer modificationsSELEX

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Area of Science:

  • Biochemistry
  • Molecular Biology
  • Biotechnology

Background:

  • Aptamers offer superior specificity and affinity compared to antibodies for molecular recognition.
  • The Systematic Evolution of Ligands by Exponential Enrichment (SELEX) process is key for aptamer development but faces challenges like nuclease susceptibility.

Purpose of the Study:

  • To review advancements in SELEX platforms and post-aptamer modifications.
  • To highlight strategies overcoming aptamer limitations and expanding their applications.

Main Methods:

  • Examination of next-generation SELEX platforms: microfluidic, capillary electrophoresis, cell-based, counter-SELEX, in vivo, and high-throughput sequencing SELEX.
  • Analysis of chemical post-aptamer modifications for enhanced stability, reduced renal filtration, and expanded target range.

Main Results:

  • Next-generation SELEX platforms offer improved efficiency and broader applicability.
  • Post-aptamer modifications significantly enhance stability, pharmacokinetics, and target recognition.

Conclusions:

  • Advanced SELEX and modifications are crucial for overcoming aptamer limitations.
  • These advancements pave the way for enhanced clinical utility in diagnostics, therapeutics, and nanotechnology.