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Electrochemical Quantification of Parathyroid Hormone Using an Aptamer-Functionalized Magnetic Nanoparticle System
Reza Didarian1, Almina Gülerman2, Veli Cengiz Özalp3
1Department of Biomedical Engineering, Ankara Yıldırım Beyazıt University, Ayvalı Mah. Takdir Cad.150 Sk, Ankara, Etlik - Keçiören, Türkiye, 06010, Turkey.
Applied Biochemistry and Biotechnology
|August 3, 2026
Summary
This study presents a new aptamer biosensor for precise parathyroid hormone (PTH) measurement during surgery. This electrochemical tool offers a sensitive and specific method for intraoperative parathormone (ioPTH) analysis, improving patient care.
Area of Science:
- Biomedical Engineering
- Analytical Chemistry
- Nanotechnology
Background:
- Accurate intraoperative parathormone (ioPTH) monitoring is crucial for parathyroid disease management.
- Conventional immunoassays for PTH analysis can be time-consuming and complex.
- There is a need for rapid, sensitive, and specific diagnostic tools for real-time ioPTH assessment.
Purpose of the Study:
- To develop a highly sensitive and specific aptamer-based electrochemical biosensor for quantitative parathyroid hormone (PTH) analysis.
- To enable reliable intraoperative parathormone (ioPTH) monitoring.
- To provide a rapid and accurate alternative to existing diagnostic methods.
Main Methods:
- Optimization of a direct electrochemical detection method for PTH on carbon screen-printed electrodes (SPEs).
- Enhancement of biosensor performance using aptamer-functionalized magnetic nanoparticles for target capture and enrichment.
- Validation of the biosensor's sensitivity, specificity, stability, and clinical accuracy using patient serum samples.
Main Results:
- Achieved a low limit of detection (LOD) of 1.9 pg/mL and a wide linear range (5-400 pg/mL).
- Demonstrated high specificity against common biological interferents and excellent long-term stability (over 75% activity for 3 months).
- Clinical validation showed a high correlation (up to 96.72%) with a commercial ELISA kit.
Conclusions:
- The developed aptamer-based electrochemical biosensor is a sensitive, specific, and stable tool for PTH quantification.
- This technology offers a promising alternative for real-time, point-of-care intraoperative parathormone (ioPTH) monitoring.
- The biosensor provides a reliable and accurate diagnostic solution, potentially improving surgical outcomes.
