Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: Apr 20, 2026

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
11:42

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

11.7K

miRNAs expression profile in zebrafish developing vessels.

Emma Ristori1, Stefania Nicoli

  • 1Yale Cardiovascular Research Center, Internal Medicine, Yale University, New Haven, CT, USA.

Methods in Molecular Biology (Clifton, N.J.)
|December 4, 2014
PubMed
Summary

This chapter details a method for profiling microRNA (miRNA) expression in developing zebrafish vessels using Illumina deep sequencing. The approach enables discovery of novel small noncoding RNAs and quantification of differentially expressed miRNAs in endothelial cells during angiogenesis.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Epidermal stem cells control periderm injury repair via matrix-driven specialization of intercellular junctions.

Nature communications·2025
Same author

Epidermal Stem Cells Control Periderm Injury Repair via Matrix-Driven Specialization of Intercellular Junctions.

bioRxiv : the preprint server for biology·2025
Same author

Genetically encoded affinity reagents are a toolkit for visualizing and manipulating endogenous protein function in vivo.

Nature communications·2025
Same author

Modeling thoracic aortic genetic variants in the zebrafish: useful for predicting clinical pathogenicity?

Frontiers in cardiovascular medicine·2025
Same author

G3BP1 ribonucleoprotein complexes regulate focal adhesion protein mobility and cell migration.

Cell reports·2025
Same author

Anti-Syndecan 2 Antibody Treatment Reduces Edema Formation and Inflammation of Murine Laser-Induced CNV.

Translational vision science & technology·2025

Area of Science:

  • Developmental Biology
  • Molecular Biology
  • Genomics

Background:

  • MicroRNAs (miRNAs) play crucial roles in vascular development and angiogenesis.
  • Understanding miRNA expression profiles in developing endothelial cells is essential for elucidating regulatory mechanisms.

Purpose of the Study:

  • To describe a detailed methodology for identifying miRNA expression profiles in developing zebrafish embryonic vessels.
  • To enable the discovery and profiling of all small noncoding RNAs, including novel miRNAs and sequence variants.

Main Methods:

  • Utilizing Illumina deep sequencing strategy for high-throughput miRNA analysis.
  • Employing Fluorescence-Activated Cell Sorting (FACS) to isolate primary endothelial cells from developing zebrafish vessels.
  • Preparing high-quality small RNA libraries using the TruSeq small RNA strategy for Illumina Hi-Seq.

More Related Videos

Imaging and 3D Reconstruction of Cerebrovascular Structures in Embryonic Zebrafish
08:00

Imaging and 3D Reconstruction of Cerebrovascular Structures in Embryonic Zebrafish

Published on: April 22, 2014

15.5K
Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis
10:00

Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis

Published on: January 12, 2015

15.3K

Related Experiment Videos

Last Updated: Apr 20, 2026

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish
11:42

Sample Preparation and Analysis of RNASeq-based Gene Expression Data from Zebrafish

Published on: October 27, 2017

11.7K
Imaging and 3D Reconstruction of Cerebrovascular Structures in Embryonic Zebrafish
08:00

Imaging and 3D Reconstruction of Cerebrovascular Structures in Embryonic Zebrafish

Published on: April 22, 2014

15.5K
Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis
10:00

Large-scale Zebrafish Embryonic Heart Dissection for Transcriptional Analysis

Published on: January 12, 2015

15.3K

Main Results:

  • The described methodology allows for comprehensive profiling of miRNA expression in endothelial cells during zebrafish development.
  • Enables identification of novel miRNA species and sequence variants.
  • Facilitates quantification of differentially expressed miRNAs in the context of angiogenesis.

Conclusions:

  • This method provides a robust platform for studying small noncoding RNA regulation in vascular development.
  • It is applicable to various model organisms and developmental stages for discovering and profiling small RNAs.
  • The technique aids in understanding the role of miRNAs in endothelial cell function and angiogenesis.