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Updated: Apr 20, 2026

The ITS2 Database
Published on: March 12, 2012
[Molecular identification of 15 species of Ilex genus based on ITS sequence analysis ]
Objective:
To establish molecular method for identification of Ilex plants using the internal transcribed spacer(ITS) bar- code.
Methods:
DNA was extracted using centrifugal columnar kits. The ITS sequences of all experimental samples were amplified and sequenced using a pair of universal primers. The alignment and the phylogenetic tree were performed in MEGA5. 0.
Results:
The length of ITS sequence in each species was 759 bp,in which there were 216 sites discrepancy.
Conclusion:
DNA barcoding technology based on ITS sequence can be used to identify plants of Ilex genus.
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