Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

DNA Microarrays02:34

DNA Microarrays

23.3K
Microarrays are high-throughput and relatively inexpensive assays that can be automated to analyze large quantities of data at a time. They are used in genome-wide studies to compare gene or protein expression under two varied conditions, such as healthy and diseased states. Microarrays consist of glass or silica slides on which probe molecules are covalently attached through surface functionalization. Most commonly, the slides are prepared through the chemisorption of silanes to silica...
23.3K
Real Time RT-PCR02:57

Real Time RT-PCR

67.7K
Real-time reverse transcription-polymerase chain reaction, or Real-time RT-PCR, is an analytical tool used to determine the expression level of target genes. The method involves converting mRNA to complementary DNA with the help of an enzyme known as reverse transcriptase, followed by the PCR amplification of the cDNA. These two processes can be performed simultaneously in a single tube or separately as a two-step reaction.
The real-time quantification of the number of amplified products is...
67.7K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Using hierarchical Bayesian modelling to assess shedder test suitability.

Forensic science international. Genetics·2026
Same author

Probabilistic searching of databases compared to binary CODIS eligibility: An improved methodology for maximizing investigative leads using short tandem repeat DNA profiles.

Forensic science international. Genetics·2026
Same author

deepNoC: A deep learning system to assign the number of contributors to a short tandem repeat DNA profile.

Forensic science international. Genetics·2026
Same author

A matter of age: Investigation of the ability to classify epithelial cells of different anatomical origin in aged samples using imaging flow cytometry.

Forensic science international·2026
Same author

STR-Uggles: Overcoming Humic Acid Inhibition Using Combined STR & qPCR Kit Chemistries.

Genes·2025
Same author

An investigation into the background levels of DNA and saliva typically detectable on penises.

International journal of legal medicine·2025
Same journal

Comparison of key diagnostics for probabilistic interpretation of STR mixture data generated with length-based and MPS methodologies.

Forensic science international. Genetics·2026
Same journal

Likelihood Ratios Given Activity-Level Propositions for DNA Transfer Evidence: Theoretical Foundations of the HaloGen Framework (Part I).

Forensic science international. Genetics·2026
Same journal

Likelihood Ratios Given Activity-Level Propositions for DNA Transfer Evidence: Practical Implementation and Simulation Studies Using the HaloGen Engine (Part II).

Forensic science international. Genetics·2026
Same journal

Forensic evaluation of 101 identity-informative SNPs in a Filipino population using massively parallel sequencing.

Forensic science international. Genetics·2026
Same journal

Reprocessing of hair roots using conventional forensic DNA analysis following RapidHIT® ID processing.

Forensic science international. Genetics·2026
Same journal

Analysis of low-input and degraded forensic DNA trace samples with NGS using the MiSeq FGx platform.

Forensic science international. Genetics·2026
See all related articles

Related Experiment Video

Updated: Apr 19, 2026

Author Spotlight: Exploring the Impact of Trauma on Cellular Aging
11:44

Author Spotlight: Exploring the Impact of Trauma on Cellular Aging

Published on: March 22, 2024

3.4K

Do low template DNA profiles have useful quantitative data?

Duncan Taylor1, John Buckleton2

  • 1Forensic Science South Australia, 21 Divett Place, Adelaide, SA 5000, Australia; School of Biological Sciences, Flinders University, GPO Box 2100, Adelaide, SA 5001, Australia.

Forensic Science International. Genetics
|December 5, 2014
PubMed
Summary
This summary is machine-generated.

Using DNA peak height information in STRmix™ analysis significantly improves likelihood ratio (LR) results, especially for low-level DNA samples. This enhancement is consistent across various template levels, confirming the value of peak height data in forensic DNA interpretation.

Keywords:
Continuous modelsDNA profile interpretationLikelihood ratiosLow templateMixtures

More Related Videos

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
06:55

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

Published on: September 24, 2015

8.8K
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

12.4K

Related Experiment Videos

Last Updated: Apr 19, 2026

Author Spotlight: Exploring the Impact of Trauma on Cellular Aging
11:44

Author Spotlight: Exploring the Impact of Trauma on Cellular Aging

Published on: March 22, 2024

3.4K
Simple Bulk Readout of Digital Nucleic Acid Quantification Assays
06:55

Simple Bulk Readout of Digital Nucleic Acid Quantification Assays

Published on: September 24, 2015

8.8K
Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies
13:24

Integration of Wet and Dry Bench Processes Optimizes Targeted Next-generation Sequencing of Low-quality and Low-quantity Tumor Biopsies

Published on: April 11, 2016

12.4K

Area of Science:

  • Forensic Science
  • Genetics
  • Molecular Biology

Background:

  • Forensic DNA profiling relies on analyzing Short Tandem Repeats (STRs).
  • Interpreting low-template DNA mixtures presents challenges due to potential allele drop-out.
  • STRmix™ software is a widely used probabilistic genotyping tool.

Purpose of the Study:

  • To evaluate the impact of incorporating DNA peak height information into STRmix™ analysis.
  • To compare the performance of STRmix™ (using peak heights) versus STRmix™ lite (semi-continuous model without peak heights).
  • To determine the utility of peak height data across different DNA template levels.

Main Methods:

  • Analysis of low-template mixed DNA profiles with known ground truths.
  • Comparison between STRmix™ V2.3 (utilizing peak heights) and STRmix™ lite (semi-continuous model).
  • Assessment of Likelihood Ratio (LR) performance under different conditions.

Main Results:

  • The use of peak height information in STRmix™ consistently increased the LR when the hypothesis (Hp) was true.
  • This improvement was most pronounced at moderate template DNA levels but was also observed at low template levels.
  • Peak height information was found to be informative even at the lowest detectable DNA levels.

Conclusions:

  • Incorporating peak height data into STRmix™ analysis enhances the discriminatory power of DNA evidence.
  • Peak height information provides valuable data for interpreting low-template DNA mixtures.
  • The findings support the continued use and development of continuous probabilistic genotyping models that leverage all available allele information.