Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Automated Microbial Diagnostics01:24

Automated Microbial Diagnostics

59
Automated diagnostic analyzers have transformed clinical microbiology by providing rapid and reliable methods for pathogen identification and antibiotic susceptibility testing. Among these systems, the Vitek 2 is widely used because it automates the traditionally labor-intensive processes of microbial identification (ID) and antibiotic susceptibility testing (AST), delivering standardized and timely results that are essential for effective patient care.Microbial Identification with ID CardsThe...
59
Physical Methods for Controlling Microbial Growth: Radiation and Filtration01:26

Physical Methods for Controlling Microbial Growth: Radiation and Filtration

1.7K
Radiation and filtration are essential tools for microbial control, targeting microorganisms through distinct mechanisms. Radiation eliminates microbes by damaging their DNA, either killing them or inhibiting their growth. Based on wavelength, radiation is classified into two types: nonionizing and ionizing radiation.Non-ionizing radiation, such as UV radiation (200–400 nm), is absorbed by DNA, causing defects that effectively disinfect surfaces, air, and water, including safety cabinets.
1.7K
Microbial Growth Measurement: Direct Methods01:23

Microbial Growth Measurement: Direct Methods

3.0K
Direct methods for measuring microbial populations in a culture are essential tools in microbiology, providing quantitative data for various applications. Among these, microscopic counts, plate counts, and serial dilution are widely used techniques, each with unique principles and applications.Microscopic CountsMicroscopic counting involves the use of a Petroff-Hausser chamber, a specialized microscope slide with a grid and defined depth. By observing a liquid culture under a microscope,...
3.0K
Key Techniques in Microbiology01:19

Key Techniques in Microbiology

3.2K
Aseptic techniques prevent contamination, ensure experimental accuracy, and protect researchers and microbial cultures. These techniques are essential in clinical, industrial, and research settings where sterility is required.Maintaining Sterility in Laboratory PracticesScientists maintain sterility by sterilizing tools with heat or chemicals, disinfecting work surfaces, and handling cultures in controlled environments. Working near an open flame or within a laminar flow hood reduces the risk...
3.2K
Antimicrobial Effectiveness01:28

Antimicrobial Effectiveness

2.1K
The effectiveness of antimicrobial agents depends on various factors influencing their ability to eliminate microbial populations. Larger microbial populations require more time for complete eradication, emphasizing the importance of population size analysis when evaluating antimicrobial efficacy.Microbial resistance to antimicrobial agents varies significantly. Highly resilient microorganisms include endospores, gram-negative bacteria, and non-enveloped viruses, while prions are exceptionally...
2.1K
Methods to Assess Microbial Populations01:30

Methods to Assess Microbial Populations

81
Assessing microbial populations is crucial for understanding microbial roles in health, ecology, and industry. Various complementary techniques—both culture-based and molecular—enable detailed analysis of microbial abundance, diversity, and function.Viable Plate CountThe viable plate count is a traditional culture-based method used to estimate the number of living microbes in a sample. After serial dilution, the sample is spread onto nutrient agar plates. Each viable cell forms a...
81

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Compounding Pharmacies for 0.5% Timolol Nasal Spray for Acute Migraine.

Missouri medicine·2025
Same author

Prescription - Transparency and the Evolution of Compounding.

International journal of pharmaceutical compounding·2025
Same author

Physical Compatibility of Cefiderocol with Selected Intravenous Drugs During Simulated Y-site Administration.

International journal of pharmaceutical compounding·2021
Same author

Serum Monitoring and Phenotype Identification of Stage I Non-Small Cell Lung Cancer Patients.

Cancer investigation·2017
Same author

Topical Metered-dosing Dispenser Performance Evaluation.

International journal of pharmaceutical compounding·2017
Same author

Adolescent and young adult women's opinions of common eating disorder prevention messages.

Eating disorders·2017

Related Experiment Video

Updated: Apr 19, 2026

Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies
09:30

Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

Published on: March 17, 2023

4.8K

Quality Control: microbial limit tests for nonsterile pharmaceuticals, part 2.

Nicole Vu, Jessica R Lou, Thomas C Kupiec

    International Journal of Pharmaceutical Compounding
    |December 6, 2014
    PubMed
    Summary

    Increasing numbers of nonsterile products are contaminated with objectionable microorganisms like bacteria, yeasts, and molds. This series examines essential microbiological quality testing for nonsterile pharmaceutical products and ingredients.

    More Related Videos

    Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate LAL Assays
    06:15

    Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate LAL Assays

    Published on: January 30, 2019

    15.7K
    Antimicrobial Characterization of Advanced Materials for Bioengineering Applications
    08:08

    Antimicrobial Characterization of Advanced Materials for Bioengineering Applications

    Published on: August 4, 2018

    23.0K

    Related Experiment Videos

    Last Updated: Apr 19, 2026

    Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies
    09:30

    Author Spotlight: Microbial Control and Monitoring Strategies for Cleanroom Environments and Cellular Therapies

    Published on: March 17, 2023

    4.8K
    Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate LAL Assays
    06:15

    Detection of Endotoxin in Nano-formulations Using Limulus Amoebocyte Lysate LAL Assays

    Published on: January 30, 2019

    15.7K
    Antimicrobial Characterization of Advanced Materials for Bioengineering Applications
    08:08

    Antimicrobial Characterization of Advanced Materials for Bioengineering Applications

    Published on: August 4, 2018

    23.0K

    Area of Science:

    • Pharmaceutical Science
    • Microbiology

    Background:

    • Nonsterile pharmaceutical products face increasing contamination issues.
    • Objectionable microorganisms, including bacteria, yeasts, and molds, are frequently identified contaminants.
    • Ensuring microbiological quality is critical for product safety and efficacy.

    Purpose of the Study:

    • To provide a comprehensive overview of microbiological quality testing for nonsterile products.
    • To address the rising concerns regarding contamination in nonsterile pharmaceuticals.
    • To consolidate information from parts 1 and 2 of this series for thorough examination.

    Main Methods:

    • Review and synthesis of microbiological quality testing methodologies.
    • Examination of common contaminants in nonsterile pharmaceutical products and ingredients.
    • Analysis of testing strategies to detect bacteria, yeasts, and molds.

    Main Results:

    • Identification of major microbial contaminants in nonsterile products.
    • Highlighting the importance of robust microbiological testing protocols.
    • Demonstration of the necessity for consistent quality control in pharmaceutical manufacturing.

    Conclusions:

    • Effective microbiological quality testing is essential to combat contamination in nonsterile products.
    • Understanding common contaminants guides the development of appropriate testing methods.
    • This series offers a foundational resource for assessing and ensuring the microbiological integrity of nonsterile pharmaceuticals.