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A sensitive and selective chemosensor for ascorbic acid based on a fluorescent nitroxide switch.

Tian Yang1, Baozhan Zheng1, Hengxing Liang2

  • 1College of Chemistry, Sichuan University, No. 29 Wangjiang Road, Chengdu, PR China.

Talanta
|December 6, 2014
PubMed
Summary
This summary is machine-generated.

A new fluorescent sensor, NAPS-NO, enables sensitive and selective detection of ascorbic acid (vitamin C). This probe recovers fluorescence upon reacting with ascorbic acid, allowing for its measurement in biological samples and living cells.

Keywords:
AsAChemosensorFluorescenceNitroxide

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Area of Science:

  • Analytical Chemistry
  • Biochemistry
  • Biomedical Engineering

Background:

  • Ascorbic acid (vitamin C) is a crucial antioxidant in biological systems.
  • Accurate detection of ascorbic acid is vital for pharmaceutical, clinical, and food industries.
  • Existing detection methods may lack sensitivity or selectivity for biological applications.

Purpose of the Study:

  • To develop a sensitive and selective fluorescent sensor for ascorbic acid detection.
  • To investigate the mechanism of fluorescence recovery upon ascorbic acid interaction.
  • To validate the sensor's utility in biological matrices and living cells.

Main Methods:

  • Synthesis and characterization of the NAPS-NO fluorescent probe.
  • Utilizing fluorescence spectroscopy and Electron Spin Resonance (ESR) to monitor the reaction.
  • Establishing a linear relationship between fluorescence intensity and ascorbic acid concentration.
  • Application of the sensor in human blood serum and cell lysate analysis.
  • Confocal laser scanning microscopy for cellular imaging.

Main Results:

  • The NAPS-NO probe exhibits fluorescence quenching by its nitroxide group.
  • Ascorbic acid rapidly reacts with the nitroxide, recovering the naphthalimide fluorescence.
  • A wide linear detection range (80 nM to 50 μM) with a low detection limit (20 nM) was achieved.
  • Successful quantification of ascorbic acid in human blood serum and HEK 293 cell lysate.
  • Demonstrated cell permeability and utility as a fluorescent probe in living cells.

Conclusions:

  • The NAPS-NO probe offers a sensitive and selective method for ascorbic acid detection.
  • The developed sensor is suitable for quantifying ascorbic acid in complex biological samples.
  • This fluorescent probe has potential for real-time monitoring of ascorbic acid in living cells.