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Development of a sporicidal test method for Clostridium difficile.

A P Fraise1, M A C Wilkinson1, C R Bradley1

  • 1Hospital Infection Research Laboratory, Queen Elizabeth Hospital, Birmingham, UK.

The Journal of Hospital Infection
|December 6, 2014
PubMed
Summary

A new method effectively evaluates disinfectant efficacy against Clostridium difficile spores, ensuring environmental safety. This standardized approach uses a 5-minute exposure time and a 5 log10 reduction pass criterion for reliable results.

Keywords:
Clostridium difficileDisinfectantTesting

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Area of Science:

  • Microbiology
  • Infectious Diseases
  • Environmental Health

Background:

  • Clostridium difficile spores are a significant source of healthcare-associated infections.
  • Current methods for evaluating disinfectants against C. difficile spores lack standardization and clinical relevance.
  • Inconsistent protocols may lead to the use of inappropriate test parameters.

Purpose of the Study:

  • To develop and validate a standardized method for assessing disinfectant activity against C. difficile spores.
  • To establish clinically relevant exposure times and pass criteria for disinfectant testing.
  • To ensure the efficacy of disinfectants used in healthcare environments.

Main Methods:

  • A joint working party adapted an enzyme-based spore purification method (Clospore method).
  • The adapted method utilizes a 5-minute exposure time.
  • A 5 log10 reduction in spore count was established as the pass criterion.

Main Results:

  • The developed method was evaluated by three independent laboratories.
  • Results demonstrated the method to be simple, repeatable, and reproducible.
  • The method yields a clean spore suspension with a high spore titre.

Conclusions:

  • The validated method provides a reliable means to assess disinfectant efficacy against C. difficile spores.
  • It is recommended that disinfectants demonstrate a 5 log10 reduction within 5 minutes under both clean and dirty conditions.
  • This standardized testing ensures disinfectants meet the requirements for environmental use in preventing C. difficile transmission.