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Published on: August 16, 2017
Identical expression profiling of human and murine TIPE3 protein reveals links to its functions
Jian Cui1, Chunyan Hao2, Wenqian Zhang1
1Department of Immunology, Shandong University School of Medicine, Ji'nan, P.R. China(JC, WZ, JS, NZ, GZ, SL)
Abstract:
Tumor necrosis factor-alpha-induced protein-8 like-3 (TNFAIP8L3, TIPE3) is a newly discovered member of TNFAIP8 family and regarded as a lipid second messenger transfer protein that promotes cancer. Yet the nature of the cells and tissues that express TIPE3 protein has not been determined. In this study, we examined TIPE3 expression in various murine and human tissues by immunohistochemistry and quantitative PCR. We found that TIPE3 expression was almost identical in most organs from human and mice. TIPE3 is a cytoplasmic protein expressed preferentially in epithelial-derived cells with secretory functions. Furthermore, TIPE3 protein is highly expressed in most human carcinoma cell lines. These results suggest that TIPE3 may play important roles in carcinogenesis and cell secretion.
Insights
Tumor necrosis factor-alpha-induced protein-8 like-3 (TIPE3) is a cytoplasmic protein found in human and mouse tissues, particularly in secretory epithelial cells. High TIPE3 expression in carcinoma cells suggests its role in cancer and secretion.
Area of Science:
- Molecular Biology
- Cell Biology
- Oncology
Background:
- Tumor necrosis factor-alpha-induced protein-8 like-3 (TNFAIP8L3, TIPE3) is a recently identified protein.
- TIPE3 is implicated as a lipid second messenger transfer protein that may promote cancer progression.
- The cellular and tissue distribution of TIPE3 protein remains largely uncharacterized.
Purpose of the Study:
- To investigate the expression patterns of TIPE3 protein in various human and murine tissues.
- To identify the specific cell types and tissues where TIPE3 is predominantly expressed.
- To explore the potential role of TIPE3 in carcinogenesis and cellular secretion.
Main Methods:
- Immunohistochemistry was employed to examine TIPE3 protein localization in tissue samples.
- Quantitative PCR (qPCR) was utilized to assess TIPE3 gene expression levels.
- Analysis included both murine and human tissue samples, as well as human carcinoma cell lines.
Main Results:
- TIPE3 protein expression patterns were found to be highly conserved between human and mouse tissues.
- TIPE3 was identified as a cytoplasmic protein with preferential expression in epithelial cells exhibiting secretory functions.
- Elevated TIPE3 protein expression was observed in a majority of human carcinoma cell lines examined.
Conclusions:
- TIPE3 exhibits a distinct expression profile, predominantly in secretory epithelial cells across species.
- The high expression of TIPE3 in carcinoma cells suggests a significant role in oncogenesis.
- TIPE3 may be involved in the regulation of cellular secretion processes, warranting further investigation.
