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Dextran grafting on PTFE surface for cardiovascular applications.
Eléonore C Michel1, Vanessa Montaño-Machado, Pascale Chevallier
1a Laboratory for Biomaterials & Bioengineering (CRC-I); Dept Min-Met-Materials Eng. & CHU de Quebec Research Center; Laval University; Quebec City, QC Canada.
Biomatter
|December 9, 2014
Summary
Grafting carboxymethyl-dextrans (CMD) onto polytetrafluoroethylene (PTFE) surfaces created non-thrombogenic biomaterials. This modification enhanced endothelial cell adhesion while limiting smooth muscle cell adhesion, suggesting versatile biomedical applications.
Area of Science:
- Biomaterials Science
- Surface Chemistry
- Biomedical Engineering
Background:
- Biomaterial surface modification with biomolecules is crucial for controlling biological responses.
- Polytetrafluoroethylene (PTFE) is a widely used biomaterial in cardiovascular applications.
- Developing advanced biomaterials with tailored surface properties is essential for improved clinical outcomes.
Purpose of the Study:
- To graft carboxymethyl-dextrans (CMD) with varying substitution degrees onto PTFE surfaces.
- To characterize the CMD coatings and confirm successful grafting.
- To evaluate the hemocompatibility and cellular adhesion properties of the modified PTFE surfaces.
Main Methods:
- Surface functionalization of PTFE with amino moieties.
- Grafting of carboxymethyl-dextrans (CMD) onto functionalized surfaces.
- Characterization of CMD polymers using FTIR, 1H-NMR, and conductimetric titration.
- Surface analysis using XPS to confirm grafting and composition.
- Hemocompatibility testing via the free hemoglobin method.
- In vitro studies on endothelial and smooth muscle cell adhesion.
Main Results:
- CMD grafting was confirmed on PTFE surfaces.
- CMD coatings exhibited non-thrombogenic properties.
- A CMD substitution degree of 0.2 significantly enhanced endothelial cell adhesion.
- The same substitution degree limited smooth muscle cell adhesion.
Conclusions:
- Carboxymethyl-dextran (CMD) modification of PTFE surfaces yields promising pro-active biomaterials.
- CMD-coated PTFE demonstrates improved hemocompatibility and selective cell adhesion properties.
- These findings suggest potential for versatile applications in cardiovascular and other biomedical fields.
