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Related Experiment Video

Updated: Apr 19, 2026

Tomato Root Transformation Followed by Inoculation with Ralstonia Solanacearum for Straightforward Genetic Analysis of Bacterial Wilt Disease
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Light modulates the root tip excision induced lateral root formation in tomato.

Sherinmol Thomas1, Yellamaraju Sreelakshmi, Rameshwar Sharma

  • 1a Repository of Tomato Genomics Resources ; Department of Plant Sciences ; University of Hyderabad ; Hyderabad , India.

Plant Signaling & Behavior
|December 9, 2014
PubMed
Summary

Removing the root tip stimulates lateral root formation (LRF) in tomato seedlings. Light exposure enhances LRF, suggesting light-modulated, shoot-derived auxin influences this process.

Keywords:
ACC, 1-Aminocyclopropane-1-carboxylic acidDAG, Days after germinationLR, Lateral rootLRF, Lateral root formationauxinethylenelateral rootslightroot tip dominancetomato

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Area of Science:

  • Plant Biology
  • Developmental Biology
  • Hormone Signaling

Background:

  • Root tips are crucial for plant growth, regulating elongation and architecture by integrating internal and external cues.
  • A signal from the root tip is thought to inhibit lateral root formation (LRF).

Purpose of the Study:

  • To investigate the role of the root tip and other plant organs in regulating lateral root formation (LRF) in tomato seedlings.
  • To determine the influence of light and auxin on LRF.

Main Methods:

  • Excision of root tips, cotyledons, and shoots from tomato seedlings.
  • Application of exogenous ethylene.
  • Observation of LRF under different light conditions.
  • Analysis of auxin accumulation in pericycle founder cells.

Main Results:

  • Root tip excision significantly induced LRF, correlating with auxin accumulation in pericycle founder cells.
  • Shoot removal completely abolished LRF, while cotyledon excision had a minor effect.
  • Exogenous ethylene did not affect LRF.
  • Seedlings exposed to light exhibited higher LRF compared to those in the dark.

Conclusions:

  • The root tip plays an inhibitory role in lateral root formation (LRF).
  • Shoot-derived factors, potentially modulated by light, are essential for LRF.
  • Light influences LRF, possibly by affecting shoot-derived auxin levels.